CXCL5 (epithelial neutrophil activating peptide-78) which acts as a potent chemoattractant and activator of neutrophil function was reported to play a multifaceted role in tumorigenesis. To investigate the role of CXCL5 in bladder cancer progression, we examined the CXCL5 expression in bladder cancer tissues by real-time PCR and Western blot, additionally, we used shRNA-mediated silencing to generate stable CXCL5 silenced bladder cancer 124 cells and defined its biological functions. Our results demonstrated that mRNA and protein of CXCL5 is increased in human bladder tumor tissues and cell lines, downregulation of CXCL5 in T24 cells resulted in significantly decreased cell proliferation, migration and increased cell apoptosis in vitro through Snail, PI3K-AKT and ERK1/2 signaling pathways. These data suggest that CXCL5 is critical for bladder tumor growth and progression, it may represent a potential application in cancer diagnosis and therapy. (c) 2014 Elsevier Inc. All rights reserved.
第一作者单位：[1]Peking Univ, Hosp 3, Dept Lab Med, Beijing 100191, Peoples R China
通讯作者：
通讯机构：[1]Peking Univ, Hosp 3, Dept Lab Med, Beijing 100191, Peoples R China[*1]Peking Univ, Hosp 3, Dept Lab Med, 49 North Garden Rd, Beijing 100191, Peoples R China
推荐引用方式(GB/T 7714)：
Zheng Jiajia,Zhu Xi,Zhang Jie.CXCL5 knockdown expression inhibits human bladder cancer T24 cells proliferation and migration[J].BIOCHEMICAL and BIOPHYSICAL RESEARCH COMMUNICATIONS.2014,446(1):18-24.doi:10.1016/j.bbrc.2014.01.172.
APA：
Zheng, Jiajia,Zhu, Xi&Zhang, Jie.(2014).CXCL5 knockdown expression inhibits human bladder cancer T24 cells proliferation and migration.BIOCHEMICAL and BIOPHYSICAL RESEARCH COMMUNICATIONS,446,(1)
MLA：
Zheng, Jiajia,et al."CXCL5 knockdown expression inhibits human bladder cancer T24 cells proliferation and migration".BIOCHEMICAL and BIOPHYSICAL RESEARCH COMMUNICATIONS 446..1(2014):18-24
