单位：[1]Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China[2]China Japan Friendship Hosp, Dept Neurol, Movement Disorder & Neurogenet Res Ctr, Beijing, Peoples R China
Multiple system atrophy (MSA) is a progressive neurodegenerative disease characterized by glial cytoplasmic inclusions containing insoluble alpha-synuclein. Since Ca(2+) plays an important role in cell degeneration, [Ca(2+)] (i) in alpha-synuclein-overexpressed human glioma cells was analyzed by Fura-2 fluorometry. Overexpression of alpha-synuclein increased the basal level of [Ca(2+)] (i) , and a higher Ca(2+) response to hydrogen peroxide was further observed. The effect that alpha-synuclein overexpression caused U251 cells to be more vulnerable to hydrogen peroxide was eliminated by Ca(2+) chelator BAPTA-AM or transient receptor potential channels blocker SKF 96365 but not by L-type Ca(2+) channel blocker nimodipine. These findings suggest that the dysregulation of cellular Ca(2+) homeostasis caused by alpha-synuclein under oxidative stress may contribute to the glial cell death in MSA.
基金：
Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2008 BAK03A02, 2007 CB914100]
第一作者单位：[1]Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China
通讯作者：
通讯机构：[1]Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China[*1]Beijing Inst Technol, Sch Life Sci, 5 Zhongguancun Nandajie, Beijing 100081, Peoples R China
推荐引用方式(GB/T 7714)：
Ying Zhengxin,Lin Fankai,Gu Weihong,et al.alpha-Synuclein increases U251 cells vulnerability to hydrogen peroxide by disrupting calcium homeostasis[J].JOURNAL of NEURAL TRANSMISSION.2011,118(8):1165-1172.doi:10.1007/s00702-011-0596-7.
APA：
Ying, Zhengxin,Lin, Fankai,Gu, Weihong,Su, Yang,Arshad, Abida...&Deng, Yulin.(2011).alpha-Synuclein increases U251 cells vulnerability to hydrogen peroxide by disrupting calcium homeostasis.JOURNAL of NEURAL TRANSMISSION,118,(8)
MLA：
Ying, Zhengxin,et al."alpha-Synuclein increases U251 cells vulnerability to hydrogen peroxide by disrupting calcium homeostasis".JOURNAL of NEURAL TRANSMISSION 118..8(2011):1165-1172
