This study aims to introduce a new pelvic reconstructive procedure and assess its safety and efficacy. Anatomic dissection was performed on ten Chinese female cadavers. The fascia 1 cm horizontally lateral to the ischial spine was firm and strong with no major vessels or nerves in the area. Thirty-two patients with stage III uterine prolapse underwent ischial spinous fascia fixation procedure. Objective and subjective results were observed in follow-up evaluations. No severe complications were observed. The objective success rate was 94% at 1-year follow-up while two patients (6.3%) showed recurrence. There were significant improvements in Aa, Ba, Ap, Bp, and C (p < 0.001) by pelvic organ prolapse quantification. Two patients suffered from lower back pain and right thigh pain, respectively, while three complained a sensation of a foreign body in the vagina. As a treatment of vaginal fornix prolapse, ischial spinous fascia fixation is found to be safe and efficacious.