单位：[1]China Acad Tradit Chinese Med, Inst Basic Res Clin Med, Beijing 100700, Peoples R China[2]China Acad Tradit Chinese Med, Inst Basic Theory, Beijing 100700, Peoples R China[3]Anhui Coll TCM, Affiliated Hosp 1, Hefei 230031, Anhui, Peoples R China[4]Sino Japan Friendship Hosp, Inst Clin Med, Beijing 100009, Peoples R China[5]Hong Kong Polytech Univ, Inst Modern Chinese Med, Shenzhen 518057, Peoples R China[6]Shanghai TCM Univ, E Inst Shanghai Municipal Educ Commiss, Shanghai, Peoples R China
To investigate the effects of ginseng polysaccharide on T-lymphocytes of enteric mucosal lymphocytes in rats with collagen induced arthritis (CIA). The peyer's patche lymphocyte (PPL), intraepithelial lymphocyte (IEL) and lamina propria lymphocyte (LPL) of SD rats were isolated. The PPL, IEL and LPL were checked with flow cytometry and these lymphocytes were co-cultured with ginseng polysaccharide separately at different dosages. ELISA measured the expression levels of TNF-alpha and IFN-gamma in supernatants. The results showed that ginseng polysaccharide could increased the protein expression of TNF-alpha and IFN-gamma in the supernatant of PPL on normal rats, and it could lower the protein expression of TNF-alpha while increase the IFN-gamma protein level in the supernatant of PPL on CIA rats. Ginseng polysaccharide could lower the protein levels both TNF-alpha and IFN-gamma in the supernatant of LPL on normal rats. And it could lower the protein levels of TNF-alpha and IFN-gamma in the supernatant of both IEL and LPL on CIA rats. Ginseng polysaccharide could decrease the expression of TNF-alpha and IFN-gamma, regulate the function of Lymphocytes in enteric mucosal immune system in CIA rats, which indicate that Ginseng polysaccharide could be used in the treatment of autoimmune disease.
基金：
National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [90209002, 3042121, 30825047]; National Scientific Support Plan [2006BAI08B01-01]; E-institutes of Shanghai Municipal Education Commission [E03008]
第一作者单位：[2]China Acad Tradit Chinese Med, Inst Basic Theory, Beijing 100700, Peoples R China
通讯作者：
通讯机构：[1]China Acad Tradit Chinese Med, Inst Basic Res Clin Med, Beijing 100700, Peoples R China[6]Shanghai TCM Univ, E Inst Shanghai Municipal Educ Commiss, Shanghai, Peoples R China
推荐引用方式(GB/T 7714)：
Zhao Hongyan,Zhang Wandong,Xiao Cheng,et al.Effect of ginseng polysaccharide on TNF-alpha and IFN-gamma produced by enteric mucosal lymphocytes in collagen induced arthritic rats[J].JOURNAL of MEDICINAL PLANTS RESEARCH.2011,5(9):1536-1542.
APA：
Zhao, Hongyan,Zhang, Wandong,Xiao, Cheng,Lu, Cheng,Xu, Shaohua...&Lu, Aiping.(2011).Effect of ginseng polysaccharide on TNF-alpha and IFN-gamma produced by enteric mucosal lymphocytes in collagen induced arthritic rats.JOURNAL of MEDICINAL PLANTS RESEARCH,5,(9)
MLA：
Zhao, Hongyan,et al."Effect of ginseng polysaccharide on TNF-alpha and IFN-gamma produced by enteric mucosal lymphocytes in collagen induced arthritic rats".JOURNAL of MEDICINAL PLANTS RESEARCH 5..9(2011):1536-1542
