Brian E. Grottkau, Xi-rui Chen, Claudia C. Friedrich, Xing-mei Yang, Wei Jing, Yao Wu, Xiao-xiao Cal, Yu-rong Liu, Yuan-ding Huang, Yun-feng Lin. DAPT Enhances the Apoptosis of Human Tongue Carcinoma Cells. International Journal of Oral Science, 1(2): 81-89, 2009 Aim To investigate the effect of DAPT (gamma-secretase inhibitor) on the growth of human tongue carcinoma cells and to determine the molecular mechanism to enable the potential application of DAPT to the treatment of tongue carcinoma. Methodology Human tongue carcinoma Tca8113 cells were cultured with DAPT. Cell growth was determined using lndigotic Reduction method. The cell cycle and apoptosis were analyzed by flow cytometry. Real-time PCR and Immuno-Fluorescence (IF) were employed to determine the intracellular expression levels. Results DAPT inhibited the growth of human tongue carcinoma Tca8113 cells by inducing G(0)-G(1) cell cycle arrest and apoptosis. The mRNA levels of Hairy/Enhancer of Split-1 (Hes-1), a target of Notch activation, were reduced by DAPT in a dose-dependent manner. Coincident with this observation, DAPT induced a dose-dependent promotion of constitutive Caspase-3 in Tca8113 cells. Conclusion DAPT may have a therapeutic value for human tongue carcinoma. Moreover, the effects of DAPT in tumor inhibition may arise partly via the modulation of Notch-1 and Caspase-3.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30801304]; Specialized Research Fund for the Doctoral Program of Higher EducationSpecialized Research Fund for the Doctoral Program of Higher Education (SRFDP) [20070610062]; State Key Laboratory of Oral Diseases, Sichuan UniversitySichuan University [SKLOD011]; Applied Fundarmental Project of Sichuan Province [2008 JY0028-2]
第一作者单位：[2]Harvard Univ, Sch Med, MassGen Hosp Children & Pediat Orthopaed, Dept Orthopaed Surg,Lab Tissue Engn, Boston, MA USA
通讯作者：
通讯机构：[1]Sichuan Univ, W China Coll Stomatol, State Key Lab Oral Dis, Chengdu 610041, Peoples R China[2]Harvard Univ, Sch Med, MassGen Hosp Children & Pediat Orthopaed, Dept Orthopaed Surg,Lab Tissue Engn, Boston, MA USA[*1]Sichuan Univ, W China Coll Stomatol, State Key Lab Oral Dis, 14,3rd Sect,Renmin S Rd, Chengdu 610041, Peoples R China
推荐引用方式(GB/T 7714)：
Grottkau Brian E.,Chen Xi-Rui,Friedrich Claudia C.,et al.DAPT Enhances the Apoptosis of Human Tongue Carcinoma Cells[J].INTERNATIONAL JOURNAL of ORAL SCIENCE.2009,1(2):81-89.doi:10.4248/ijos.08025.
APA：
Grottkau, Brian E.,Chen, Xi-Rui,Friedrich, Claudia C.,Yang, Xing-mei,Jing, Wei...&Lin, Yun-feng.(2009).DAPT Enhances the Apoptosis of Human Tongue Carcinoma Cells.INTERNATIONAL JOURNAL of ORAL SCIENCE,1,(2)
MLA：
Grottkau, Brian E.,et al."DAPT Enhances the Apoptosis of Human Tongue Carcinoma Cells".INTERNATIONAL JOURNAL of ORAL SCIENCE 1..2(2009):81-89
医学