单位：[1]Huazhong Univ Sci & Technol, Dept Microbiol, Sch Basic Med Sci, Tongji Med Coll, Wuhan 430030, Peoples R China[2]China Japan Friendship Hosp, Beijing 100029, Peoples R China
In this study, by analysis of genome structures of E. coli, the relationships between the genomic types of E. coli and the associated diseases were investigated. Samples of sputum, urine and other excretions from patients with different infective diseases were collected. And 62 E. coli strains were isolated from these samples. Intact bacterial genomic DNA was cleaved with I-CeuI, separated by pulsed field gel electrophoresis and then typed on the basis of cleavage map. The results showed that 7 I-CeuI sites were found in all the genome structures of the 62 E. coli, indicating that there were 7 rrn operons in the genomes. The size of genome ranged from 4500 kb to 5000 kb. According to the genome structures, 62 E. coli strains were divided into 30 genome types. It was concluded that genome structures of E. coli isolated from the patients with different infective diseases varied to some extent, suggesting that some genome types of E. coli were closely related to some infective diseases.
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第一作者：
第一作者单位：[2]China Japan Friendship Hosp, Beijing 100029, Peoples R China
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推荐引用方式(GB/T 7714)：
Yi Meiying,Liu Ruen,Huang Hanju.Relationship between Genomic Types of Escherichia coli and Clinical Diseases[J].JOURNAL of HUAZHONG UNIVERSITY of SCIENCE and TECHNOLOGY-MEDICAL SCIENCES.2008,28(4):383-385.doi:10.1007/s11596-008-0403-3.
APA：
Yi, Meiying,Liu, Ruen&Huang, Hanju.(2008).Relationship between Genomic Types of Escherichia coli and Clinical Diseases.JOURNAL of HUAZHONG UNIVERSITY of SCIENCE and TECHNOLOGY-MEDICAL SCIENCES,28,(4)
MLA：
Yi, Meiying,et al."Relationship between Genomic Types of Escherichia coli and Clinical Diseases".JOURNAL of HUAZHONG UNIVERSITY of SCIENCE and TECHNOLOGY-MEDICAL SCIENCES 28..4(2008):383-385
