The methods for detecting and typing human papillomavirus (HPV) in most molecular epidemiological surveys of verrucae vulgaris were based on PCR followed by sequencing or hybridization. However. the amplification efficacies of different assays for the detection of HPV DNAs varied largely. In this study, a novel multiplex PCR method to detect and type the HPVs (HPV-1, -2, -27 and -57) related to verrucae vulgaris was described. This method allows detecting and typing HPV DNA simultaneously in one reaction based on the length of the PCR products after electrophoresis. The sensitivity and specificity of this multiplex PCR method was assessed with the standard template panels and the spiking sample panels, and evaluated with the clinical samples, compared with PCR assay with primer MY09/11. The results showed the novel method had reliable clinical sensitivity (97.6%) and specificity (100%), significantly higher than that of the PCR using consensus primer, MY09/11. In addition, this method can effectively detect multiple HPV infection within the lesions. This simplified, economic and time-saving multiplex PCR method provides a useful additional tool for the clinical epidemiological study of verrucae vulgaris. (C) 2007 Elsevier B.V. All rights reserved.
语种：
外文
被引次数：
WOS：
中科院(CAS)分区：
出版当年[2007]版：
大类|3 区医学
最新[2025]版：
大类|4 区医学
小类|4 区生化研究方法4 区生物工程与应用微生物4 区病毒学
JCR分区：
出版当年[2006]版：
Q2BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3BIOCHEMICAL RESEARCH METHODSQ4VIROLOGY
最新[2023]版：
Q3BIOCHEMICAL RESEARCH METHODSQ3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3VIROLOGY
第一作者单位：[1]Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 100052, Peoples R China[2]Xian Jiaotong Univ, Sch Med, Xian 710061, Peoples R China
通讯作者：
通讯机构：[1]Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 100052, Peoples R China[*1]Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Ying Xin Rd 100, Beijing 100052, Peoples R China
推荐引用方式(GB/T 7714)：
Lei Yan-Jun,Gao Chen,An Run,et al.Development of a multiplex PCR method for detecting and typing human papillomaviruses in verrucae vulgaris[J].JOURNAL of VIROLOGICAL METHODS.2008,147(1):72-77.doi:10.1016/j.jviromet.2007.08.005.
APA：
Lei, Yan-Jun,Gao, Chen,An, Run,Shi, Qi,Chen, Jian-Ming...&Dong, Xiao-Ping.(2008).Development of a multiplex PCR method for detecting and typing human papillomaviruses in verrucae vulgaris.JOURNAL of VIROLOGICAL METHODS,147,(1)
MLA：
Lei, Yan-Jun,et al."Development of a multiplex PCR method for detecting and typing human papillomaviruses in verrucae vulgaris".JOURNAL of VIROLOGICAL METHODS 147..1(2008):72-77
