单位：[1]Chinese Acad Med Sci, Inst Basic Med Sci, Beijing 100005, Peoples R China[2]Chinese Acad Med Sci, Peking Union Med Coll, Sch Basic Med, Dept Pharmacol, Beijing 100005, Peoples R China[3]China Japan Friendship Hosp, Inst Clin Res, Dept Immunol & Tissue Engn, Beijing 100029, Peoples R China
To determine the survival and differentiation of cultured Human amniotic cells (HACs) upon transplantation into the brain of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)induced Parkinson disease (PD) mice. Mouse model of PD was established with injections of MPTP (15 mg/kg, fourth, 2 h interval). After being labeled with PKH26, HACs isolated from human were transplanted into the striatum of PD mice. Immunohistochemistry was performed to evaluate the toxicity of MPTP in the substantia. nigra, graft survival and endogenous neurogenesis. Brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) level in the striatum were tested by ELISA. Our results showed that cultured HACs can express the marker of neural progenitor cells and differentiate into neuron, dopaminergic neuron, astrocyte and oligodendrocyte. TH-positive neural cells were significantly reduced in the substantia. nigra in the model mice, whereas which increased in transplantation mice. Immunohistology results showed that transplanted HACs survived and migrated in the brain of PD model mouse, though no morphological integration was observed. BrdU-positive cells in the Subventricular zone (SVZ) and neurotrophins of the striatum increased in the transplantation mice. The results suggested that transplanted HACs could survive and promote the endogenous neurogenesis of mice, which maybe related to the increased level of neurotrophins of the striatum. (C) 2008 Elsevier B.V. All rights reserved.
第一作者单位：[1]Chinese Acad Med Sci, Inst Basic Med Sci, Beijing 100005, Peoples R China[2]Chinese Acad Med Sci, Peking Union Med Coll, Sch Basic Med, Dept Pharmacol, Beijing 100005, Peoples R China
通讯作者：
通讯机构：[3]China Japan Friendship Hosp, Inst Clin Res, Dept Immunol & Tissue Engn, Beijing 100029, Peoples R China[*1]Chinese Acad Med Sci, Peking Union Med Coll, Sch Basic Med, Dept Pharmacol, 5 Dongdan Santiao, Beijing 100005, Peoples R China
推荐引用方式(GB/T 7714)：
Kong Xiang-Ying,Cai Zhe,Pan Lin,et al.Transplantation of human amniotic cells exerts neuroprotection in MPTP-induced Parkinson disease mice[J].BRAIN RESEARCH.2008,1205:108-115.doi:10.1016/j.brainres.2008.02.040.
APA：
Kong, Xiang-Ying,Cai, Zhe,Pan, Lin,Zhang, Lan,Shu, Jun...&Zuo, Ping-Ping.(2008).Transplantation of human amniotic cells exerts neuroprotection in MPTP-induced Parkinson disease mice.BRAIN RESEARCH,1205,
MLA：
Kong, Xiang-Ying,et al."Transplantation of human amniotic cells exerts neuroprotection in MPTP-induced Parkinson disease mice".BRAIN RESEARCH 1205.(2008):108-115
