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Cloning and promoter activity of rat Smad1 5'-flanking region in rat hepatic stellate cells

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收录情况： ◇ SCIE

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单位： [1]Univ Manitoba, Fac Pharm, Winnipeg, MB R3T 2N2, Canada [2]China Japan Friendship Hosp, Dept Pharmacol, Beijing, Peoples R China [3]Univ Manitoba, Fac Med, Dept Physiol, Winnipeg, MB R3T 2N2, Canada [4]Univ Manitoba, Fac Med, Dept Internal Med, Sect Hepatol, Winnipeg, MB R3T 2N2, Canada [*1]Univ Manitoba, Fac Pharm, 50 Sifton Rd, Winnipeg, MB R3T 2N2, Canada

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关键词： Smad1 hepatic stellate cell promoter cloning

摘要：

Smad1 is an important signaling molecule for members of transforming growth factor-beta (TGF-beta) superfamily. Increased expression of Smad1 in activated hepatic stellate cells (HSCs) indicates a role of Smad1 in liver fibrosis. Therefore, understanding of Smad1 gene expression could be important to control the activation of HSCs. Current study reports the cloning and characterizing rat Smad1 5'-flanking region in liver cells. Rat Smad1 5'-flanking region was cloned by PCR method. Promoter deletional analysis and electrophoretic mobility shift assay (EMSA) were examined in hepatocyte and HSCs cell line (CFSC-8B cells), respectively. Results indicated that rat Smad1 used GC-box as its promoter and there was a transcriptional regulatory element located at the region of -163 to -56bp. EMSA demonstrated two bands on Smad1 promoter region. Smad1 promoter activity was higher in CFSC-8B cells cultured on uncoated plastic dish than that of CFSC-8B cells cultured on Matrigel-coated plastic dish. In conclusion, rat Smad1 promoter was cloned and characterized in hepatocyte and HSC cell line (CFSC-8B cells) at different culture conditions.

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出版当年[2006]版：

大类 | 4 区生物

最新[2025]版：

大类 | 3 区生物学

小类 | 3 区细胞生物学

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出版当年[2005]版：

Q3 CELL BIOLOGY

最新[2023]版：

Q3 CELL BIOLOGY

影响因子： 最新[2023版] 3.5 最新五年平均[2021-2025] 3.3 出版当年[2005版] 1.681 出版当年五年平均[2001-2005] 0 出版前一年[2004版] 1.714 出版后一年[2006版] 1.862

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通讯机构： [*1]Univ Manitoba, Fac Pharm, 50 Sifton Rd, Winnipeg, MB R3T 2N2, Canada

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