Kinetics of the endocytotic pathway of Low Density Lipoprotein (LDL) in human endothelial cells line under shear stress: An in vitro confocal microscopy study
We studied the effect of mechanical forces (shear stress) on the kinetics of internalization of native LDL and ox-LDL in endothelial cell line ECV304. This study was performed by using Confocal microscopy and FRET with two carbocyanine dyes, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiO) as the donor and 3,3'-dioctadecyloxacarbocyanine perchlorate (DiI) as the receptor. The cells were incubated with a culture medium containing either 10 mu g/ml DiI-LDL or DiO-LDL in static conditions or subjected to a laminar flow under a Confocal Laser Scanning Microscope (SP2 Leica, Germany). The results showed: (1) the possibility to evaluate the kinetics of LDL endocytosis in living cells, (2) shear stress in comparison with control group more effectively enhanced LDL uptake, (3) ox-LDL (> 50 mu g/ml) > 4 hours incubation was found to affect the cells as reflected by their detachment at low shear stress.
第一作者单位：[*1]Fac Med, CNRS, UMR 7536 LEMTA, Grp Mecan & Ingn Cellulaire & Tissulaire, BP 184, F-54500 Vandoeuvre Les Nancy, France
通讯作者：
通讯机构：[*1]Fac Med, CNRS, UMR 7536 LEMTA, Grp Mecan & Ingn Cellulaire & Tissulaire, BP 184, F-54500 Vandoeuvre Les Nancy, France
推荐引用方式(GB/T 7714)：
Traore M,Sun RJ,Fawzi-Grancher S,et al.Kinetics of the endocytotic pathway of Low Density Lipoprotein (LDL) in human endothelial cells line under shear stress: An in vitro confocal microscopy study[J].CLINICAL HEMORHEOLOGY and MICROCIRCULATION.2005,33(3):243-251.
APA：
Traore, M,Sun, RJ,Fawzi-Grancher, S,Dumas, D,Qing, X...&Muller, S.(2005).Kinetics of the endocytotic pathway of Low Density Lipoprotein (LDL) in human endothelial cells line under shear stress: An in vitro confocal microscopy study.CLINICAL HEMORHEOLOGY and MICROCIRCULATION,33,(3)
MLA：
Traore, M,et al."Kinetics of the endocytotic pathway of Low Density Lipoprotein (LDL) in human endothelial cells line under shear stress: An in vitro confocal microscopy study".CLINICAL HEMORHEOLOGY and MICROCIRCULATION 33..3(2005):243-251
