单位：[1]Department of Urology, Beijing Friendship Hospital, Capital Medical University,Beijing 100050, China临床科室泌尿外科泌尿外科首都医科大学附属北京友谊医院[2]Department of Stomatology, Beijing ShijitanHospital, Capital Medical University, Beijing 100038, China[3]Departmentof Internal Medicine, Division of Nephrology, Leiden University Medical Center,Leiden 2333 ZA, The Netherlands[4]Department of Biological Repositories,Zhongnan Hospital of Wuhan University, Wuhan 430071, China
BackgroundSmall nucleolar RNAs (snoRNAs) are a new non-coding RNAs (ncRNAs), which have not been widely investigated and are identified to be involved in tumorigenesis. But the function of snoRNAs in BLCA has not been reported yet.MethodsSnoRNAs signature (SNORS) was constructed through LASSO cox regression analysis. Integrated analysis of candidate snoRNAs was performed to detect the correlation between copy number variation (CNV)/DNA methylation/protein/mRNA/alternative splicing (AS). Then we built a nomogram integrating independent prognostic factors to assist the clinical utility.ResultsWe have screened out 15 prognostic differentially expressed snoRNAs (DESs) and constructed SNORS consisting of 5 candidate snoRNAs which could appropriately stratify patients into low or high SNORS groups with distinct prognosis. Then we found 5 candidate snoRNAs might be regulated by their own CNV and DNA methylation. Moreover, 5 candidate snoRNAs were significantly correlated mRNA and alternative splicing (AS), which might regulate diverse biological process in tumorigenesis, such as "extracellular matrix", "epithelial-mesenchymal transition (EMT)", etc. signaling pathways. Furthermore, SNORS was an independent prognostic factor, which was strikingly correlated with clinical outcome. Through inporating with other variables, we have established a predictive nomogram, which was more effectively to predict prognosis than any other variables alone.ConclusionOur findings first highlighted an important role of snoRNAs in BLCA and established a potential prognostic model which could serve as a biomarker for BLCA.
基金：
Beijing Postdoctoral Research FoundationChina Postdoctoral Science Foundation [ZZ2019-04]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81802550]; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2019M660041]
第一作者单位：[1]Department of Urology, Beijing Friendship Hospital, Capital Medical University,Beijing 100050, China
通讯作者：
推荐引用方式(GB/T 7714)：
Cao Rui,Ma Bo,Yuan Lushun,et al.Small nucleolar RNAs signature (SNORS) identified clinical outcome and prognosis of bladder cancer (BLCA)[J].CANCER CELL INTERNATIONAL.2020,20(1):doi:10.1186/s12935-020-01393-7.
APA：
Cao Rui,Ma Bo,Yuan Lushun,Wang Gang&Tian Ye.(2020).Small nucleolar RNAs signature (SNORS) identified clinical outcome and prognosis of bladder cancer (BLCA).CANCER CELL INTERNATIONAL,20,(1)
MLA：
Cao Rui,et al."Small nucleolar RNAs signature (SNORS) identified clinical outcome and prognosis of bladder cancer (BLCA)".CANCER CELL INTERNATIONAL 20..1(2020)
