单位：[1]China‐Japan Friendship School of Clinical Medicine, Peking University, Beijing, China[2]Department of Urology, China‐Japan Friendship Hospital, Beijing, China[3]College of Psychology, North China University of Science and Technology, Tangshan, China
Aim To explore the molecular mechanism of nonmuscle invasive bladder cancer (NMIBC), matched normal, and cancer tissues of 10 NMIBC were examined for RNA sequencing. Methods We profiled the messenger RNA (mRNA) and long noncoding RNA (lncRNA) expression of patients with NMIBC. Differentially expressed mRNAs and lncRNAs were screened between cancer and normal tissues and validated by quantitative polymerase chain reaction (qPCR), and lncRNA-mRNA-miRNA interaction network was constructed. Results A total of 91 upregulated and 190 downregulated genes and 34 upregulated and 58 downregulated lncRNAs were screened from the sequencing result. The differentially expressed mRNAs were enriched in focal adhesion, rap1 signaling pathway, Hippo signaling pathway, PI3K-Akt signaling pathway, extracellular matrix (ECM)-receptor interaction, Ras signaling pathway, and mitogen-activated protein kinases signaling pathway, of which some pathways were involved in the cancer development. In the RNA sequencing, KIT and laminin subunit gamma gamma 3 (LAMC3) were significantly downregulated in the NMIBC group compared with the normal group. The results of quantitative reverse transcription PCR showed that the expression of LAMC3 and KIT were significantly decreased in the NMIBC group compared with the normal group. The lncRNA-mRNA-miRNA interaction network was constructed by Cytoscape software to further investigate the interaction correlations. The results implied that KIT and LAMC3 might regulate the lncRNAs (such as ENST00000445707, ENST00000501122, ENST00000505254, ENST00000528986, ENST00000557661, ENST00000602964, ENST00000614517, ENST00000620864, and ENST00000623414) by the miRNAs (such as hsa-let-7f-2-3p, hsa-miR-125a-3p, hsa-miR-134-3p, hsa-miR-191-5p, hsa-miR-210-5p, hsa-miR-30a-5p, hsa-miR-30d-5p, hsa-miR-30e-5p, hsa-miR-92a-2-5p, and hsa-miR-95-3p), and finally played a role in the development of NMIBC cancer. Conclusion Altogether, our study preliminarily indicated that KIT and LAMC3 might play a crucial role in the development of NMIBC cancer via a complex mRNA-lncRNA-miRNA regulatory network.
基金：
International S&T Cooperation Program of China [2014DFE30010]
第一作者单位：[1]China‐Japan Friendship School of Clinical Medicine, Peking University, Beijing, China[2]Department of Urology, China‐Japan Friendship Hospital, Beijing, China
共同第一作者：
通讯作者：
通讯机构：[1]China‐Japan Friendship School of Clinical Medicine, Peking University, Beijing, China[2]Department of Urology, China‐Japan Friendship Hospital, Beijing, China[*1]Department of Urology, China‐Japan Friendship Hospital, 2 Yinghua East Street, Chaoyang 100020, Beijing 100029, China.
推荐引用方式(GB/T 7714)：
He Yu-hui,Deng Yi-sen,Peng Pan-xin,et al.A novel messenger RNA and long noncoding RNA signature associated with the progression of nonmuscle invasive bladder cancer[J].JOURNAL of CELLULAR BIOCHEMISTRY.2019,120(5):8101-8109.doi:10.1002/jcb.28089.
APA：
He, Yu-hui,Deng, Yi-sen,Peng, Pan-xin,Wang, Ning,Wang, Jian-feng...&Zhou, Xiao-feng.(2019).A novel messenger RNA and long noncoding RNA signature associated with the progression of nonmuscle invasive bladder cancer.JOURNAL of CELLULAR BIOCHEMISTRY,120,(5)
MLA：
He, Yu-hui,et al."A novel messenger RNA and long noncoding RNA signature associated with the progression of nonmuscle invasive bladder cancer".JOURNAL of CELLULAR BIOCHEMISTRY 120..5(2019):8101-8109
