单位：[a]Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, Beijing, 100081, China[b]International Medical Center, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China首都医科大学附属北京友谊医院[c]Department of Chemistry, School of Materials Science and Chemical Engineering, Ningbo University, Ningbo, 315211, China
Luminescence lifetime imaging (LLIM) holds great promise in biomedical research owing to its excellent sensitivity and remarkable temporal-spatial resolution, but the development of effective long-lived luminescent probes for LLIM remains an unmet need. Herein, we designed two water-dispersible europium-doped nanoparticles (Eu-D1 and Eu-D2 NPs) for cellular LLIM, showing intense red luminescence emission and an increased lifetime of 4 orders of magnitude (similar to 56 000 times) than undoped NPs, which could be attributed to the multiple manipulations of their aggregation states, including efficient Forster resonance energy transfer (FRET), suppressed self-quenching, and restrained solvent effects. With good bioavailability, the as-developed NPs were demonstrated as reliable candidates for long-lifetime imaging agents. Such a simple and versatile strategy could be extended to develop various luminescent nanoprobes for advanced high-precision bioimaging.
基金：
Beijing Institute of Technology Research Fund Program for Young Scholars; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [51603088, 91859123]; K. C. Wong Magna Fund in Ningbo University
第一作者单位：[a]Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, Beijing, 100081, China
共同第一作者：
通讯作者：
通讯机构：[a]Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, Beijing, 100081, China[c]Department of Chemistry, School of Materials Science and Chemical Engineering, Ningbo University, Ningbo, 315211, China[*1]Department of Chemistry, School of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, P. R. China[*2]Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, Beijing 100081, P. R. China
推荐引用方式(GB/T 7714)：
Fang F,Zhao D,Zhang Y,et al.Europium-Doped Nanoparticles for Cellular Luminescence Lifetime Imaging via Multiple Manipulations of Aggregation State[J].ACS APPLIED BIO MATERIALS.2020,3(8):5103-5110.doi:10.1021/acsabm.0c00580.
APA：
Fang, F,Zhao, D,Zhang, Y,Li, M,Ye, J&Zhang, J.(2020).Europium-Doped Nanoparticles for Cellular Luminescence Lifetime Imaging via Multiple Manipulations of Aggregation State.ACS APPLIED BIO MATERIALS,3,(8)
MLA：
Fang, F,et al."Europium-Doped Nanoparticles for Cellular Luminescence Lifetime Imaging via Multiple Manipulations of Aggregation State".ACS APPLIED BIO MATERIALS 3..8(2020):5103-5110
