单位：[1]State Key Laboratory of Heavy Oil Processing, China University of Petroleum-Beijing, Beijing 102249, China[2]Department of Orthopaedics, Tianjin Hospital, Tianjin 300211, China[3]Department of Cardiology, Beijing Friendship Hospital, Capital Medical University, Beijing 10050, China临床科室心血管中心心内科首都医科大学附属北京友谊医院[4]Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
Aristolochic acid analogues (AAAs), naturally existing in herbal Aristolochia and Asarum genera, were once widely used in traditional pharmacopeias because of their anti-inflammatory properties, but lately they were identified as potential nephrotoxins and mutagens. A method for rapid characterization of AAAs in serum was developed using ion mobility spectrometry coupled with mass spectrometry (IMS-MS). Five AAAs, containing four aristolochic acids and one aristolactam, were separated and identified within milliseconds. AAAs were separated in gas phase based on the difference of their ion mobility (K-0), and then identified based on their K-0 values, m/z, and product ions from MS/MS. Quantitative analysis of AAAs was performed using an internal standard with a satisfactory sensitivity. Limits of detection (signal-to-noise = 3) and quantification (signal-to-noise = 10) were 1-5 ng/mL and 3-8 ng/mL, respectively. The method was validated and successfully applied to the pharmacokinetics study of AAAs in rats, offering a promising way for fast screening and evaluation of AAAs in biological samples.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21874153]; Science Foundation of China University of Petroleum, Beijing [2462017BJB09]; Tianjin Municipal Health Bureau [16KG143]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|3 区化学
小类|2 区分析化学3 区生化研究方法
最新[2025]版：
大类|2 区化学
小类|3 区生化研究方法3 区分析化学
JCR分区：
出版当年[2019]版：
Q1BIOCHEMICAL RESEARCH METHODSQ1CHEMISTRY, ANALYTICAL
最新[2023]版：
Q1BIOCHEMICAL RESEARCH METHODSQ1CHEMISTRY, ANALYTICAL
第一作者单位：[1]State Key Laboratory of Heavy Oil Processing, China University of Petroleum-Beijing, Beijing 102249, China
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推荐引用方式(GB/T 7714)：
Yanfen Zhang,Yehua Han,Chenglong Dong,et al.Rapid characterization and pharmacokinetic study of aristolochic acid analogues using ion mobility mass spectrometry[J].ANALYTICAL and BIOANALYTICAL CHEMISTRY.2021,413(16):4247-4253.doi:10.1007/s00216-021-03371-y.
APA：
Yanfen Zhang,Yehua Han,Chenglong Dong,Chen Li,Tuo Liang...&Honggang Nie.(2021).Rapid characterization and pharmacokinetic study of aristolochic acid analogues using ion mobility mass spectrometry.ANALYTICAL and BIOANALYTICAL CHEMISTRY,413,(16)
MLA：
Yanfen Zhang,et al."Rapid characterization and pharmacokinetic study of aristolochic acid analogues using ion mobility mass spectrometry".ANALYTICAL and BIOANALYTICAL CHEMISTRY 413..16(2021):4247-4253
