单位：[1]Department of Oral and Maxillofacial-Head and Neck Oncology, Beijing Stomatological Hospital, Capital Medical University, Beijing, China[2]Department of Stomatology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, China[3]Department of Otorhinolaryngology-Head and Neck Surgery, Peking University First Hospital, Beijing, China[4]Clinical Epidemiology and EBM Unit, National Clinical Research Center for Digestive Diseases, Beijing Friendship Hospital, Capital Medical University, Beijing, China医技科室北京市临床医学研究所国家消化中心（方法学平台）首都医科大学附属北京友谊医院
Objective Lymph node metastasis is the most important factor influencing the prognosis of oral squamous cell carcinoma (OSCC) patients. However, there is no proper method for predicting lymph node metastasis. This study aimed to construct and validate a preoperative prediction model for lymph node metastasis and guide personalized neck management based on the gene expression profile and clinicopathological parameters of OSCC. Methods Based on a previous study of related genes in OSCC, the mRNA expression of candidate genes was evaluated by real-time PCR in OSCC specimens. In this retrospective study, the gene expression profile and clinicopathological parameters of 112 OSCC patients were combined to construct the best prediction model for lymph node metastasis of OSCC. The model was validated with 95 OSCC samples in this study. Logistic regression analysis was used. The area under the curve (AUC) ultimately determined the diagnostic value of the prediction model. Results The two genes CDKN2A + PLAU were closely related to lymph node metastasis of oral squamous cell carcinoma. The model with the combination of CDKN2A, PLAU, T stage and pathological grade was the best in predicting lymph node metastasis (AUC = 0.807, 95% CI: 0.713-0.881, P=0.0001). The prediction model had a specificity of 96% and sensitivity of 72.73% for stage T1 and T2 OSCC (AUC = 0.855, 95% CI: 0.697-0.949, P=0.0001). Conclusions High expression of CDKN2A and PLAU was associated with lymph node metastasis in OSCC. The prediction model including CDKN2A, PLAU, T stage and pathological grade can be used as the best diagnostic model for lymph node metastasis in OSCC.
基金：
Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support [XMLX201819]; Capital's Funds for Health Improvement and Research [CFH2020-2-2143]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [82072984]; Program of Beijing Municipal Education commission [KM202110025008]; Shanghai Science and Technology CommitteeShanghai Science & Technology Committee [18DZ2291500]
第一作者单位：[1]Department of Oral and Maxillofacial-Head and Neck Oncology, Beijing Stomatological Hospital, Capital Medical University, Beijing, China[2]Department of Stomatology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, China
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推荐引用方式(GB/T 7714)：
Wang Shu,Li Tiancheng,Liu Huan,et al.A Combined Prediction Model for Lymph Node Metastasis Based on a Molecular Panel and Clinicopathological Factors in Oral Squamous Cell Carcinoma[J].FRONTIERS in ONCOLOGY.2021,11:doi:10.3389/fonc.2021.660615.
APA：
Wang, Shu,Li, Tiancheng,Liu, Huan,Wei, Wei,Yang, Yang...&Feng, Zhien.(2021).A Combined Prediction Model for Lymph Node Metastasis Based on a Molecular Panel and Clinicopathological Factors in Oral Squamous Cell Carcinoma.FRONTIERS in ONCOLOGY,11,
MLA：
Wang, Shu,et al."A Combined Prediction Model for Lymph Node Metastasis Based on a Molecular Panel and Clinicopathological Factors in Oral Squamous Cell Carcinoma".FRONTIERS in ONCOLOGY 11.(2021)
