单位：[1]Department of Pulmonary and Critical Care Medicine, Center of Respiratory Medicine, China-Japan Friendship Hospital, Beijing, China,[2]Graduate School of Peking Union Medical College, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China,[3]National Center for Respiratory Medicine, Beijing, China,[4]Institute of Respiratory Medicine, Chinese Academy of Medical Sciences, Beijing, China,[5]National Clinical Research Center for Respiratory Diseases, Beijing, China,[6]Department of Pulmonary and Critical Care Medicine, First Hospital of China Medical University, Shenyang, China,[7]Department of Pulmonary and Critical Care Medicine, Fourth Hospital of China Medical University, Shenyang, China
Purpose Chronic obstructive pulmonary disease (COPD) is a complex and persistent lung disease and lack of biomarkers. The aim of this study is to screen and verify effective biomarkers for medical practice. Methods Differential expressed genes analysis and weighted co-expression network analysis were used to explore potential biomarker. Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and Gene set enrichment analysis (GSEA) analysis were used to explore potential mechanism. CIBERSORTx website was used to evaluate tissue-infiltrating immune cells. Enzyme-linked immunosorbent assay (ELISA) was used to assess the concentrations of the Lp-PLA2 in serum. Results Ten genes were selected via combined DEGs and WGCNA. Furthermore, PLA2G7 was choose based on validation from independent datasets. Immune infiltrate and enrichment analysis suggest PLA2G7 may regulate immune pathway via macrophages. Next, Lp-PLA2(coded by PLA2G7 gene) level was upregulated in COPD patients, increased along with The Global Average of COPD (GOLD) stage. In additional, Lp-PLA2 level was significant correlate with FEV1/FVC, BMI, FFMI, CAT score, mMRC score and 6MWD of COPD patients. Finally, the predictive efficiency of Lp-PLA2 level (AUC:0.796) and derived nomogram model (AUC:0.884) in exercise tolerance was notably superior to that of the sit-to-stand test and traditional clinical features. Conclusion Lp-PLA2 is a promising biomarker for COPD patients and is suitable for assessing exercise tolerance in clinical practice.
基金：
Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences [2020-PT320-001]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81900040, 81700041]
第一作者单位：[1]Department of Pulmonary and Critical Care Medicine, Center of Respiratory Medicine, China-Japan Friendship Hospital, Beijing, China,[2]Graduate School of Peking Union Medical College, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China,[3]National Center for Respiratory Medicine, Beijing, China,[4]Institute of Respiratory Medicine, Chinese Academy of Medical Sciences, Beijing, China,[5]National Clinical Research Center for Respiratory Diseases, Beijing, China,
共同第一作者：
通讯作者：
通讯机构：[1]Department of Pulmonary and Critical Care Medicine, Center of Respiratory Medicine, China-Japan Friendship Hospital, Beijing, China,[2]Graduate School of Peking Union Medical College, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China,[3]National Center for Respiratory Medicine, Beijing, China,[4]Institute of Respiratory Medicine, Chinese Academy of Medical Sciences, Beijing, China,[5]National Clinical Research Center for Respiratory Diseases, Beijing, China,
推荐引用方式(GB/T 7714)：
Mingming Deng,Yan Yin,Qin Zhang,et al.Identification of Inflammation-Related Biomarker Lp-PLA2 for Patients With COPD by Comprehensive Analysis[J].FRONTIERS in IMMUNOLOGY.2021,12:doi:10.3389/fimmu.2021.670971.
APA：
Mingming Deng,Yan Yin,Qin Zhang,Xiaoming Zhou&Gang Hou.(2021).Identification of Inflammation-Related Biomarker Lp-PLA2 for Patients With COPD by Comprehensive Analysis.FRONTIERS in IMMUNOLOGY,12,
MLA：
Mingming Deng,et al."Identification of Inflammation-Related Biomarker Lp-PLA2 for Patients With COPD by Comprehensive Analysis".FRONTIERS in IMMUNOLOGY 12.(2021)
医学