单位：[1]Liver Transplantation Center, National Clinical Research Center for Digestive Diseases and Beijing Key Laboratory of Tolerance Induction and Organ Protection in Transplantation, Beijing Friendship Hospital, Capital Medical University, Beijing, China临床科室国家中心普外分中心普外四科（肝脏移植外科）首都医科大学附属北京友谊医院[2]Department of General Surgery, Jiaozuo People’s Hospital, Xinxiang Medical University, Jiaozuo, China[3]Department of Immunology, Tianjin Key Laboratory of Cellular and Molecular Immunology, Tianjin Medical University, Tianjin, China
Highly upregulated in liver cancer (HULC) had a significant predictive effect on tumor growth and metastasis of hepatocellular carcinoma (HCC); however, the mechanisms of HULC on HCC still need to be clarified. We attempted to determine the roles of HULC and miR-107 in autophagy and invasion of HCC. HULC siRNA reduced the level of autophagy. The impact of HULC siRNA on invasion can be reversed by activating autophagy in HCC cell lines. Further studies on HULC and autophagy were conducted. An interacting sequence between HULC and miR-107, as well as miR-107 and Atg12, was predicted by software. The relationship of each pair of molecules was confirmed by luciferase reporter assays. The negative impacts of miR-107 on autophagy and invasion were proved in HCC cell lines. The inhibitor of miR-107-promoted invasion can also be reversed by Atg12 siRNA. The changes of miR-107, Atg12, epithelial-mesenchymal transition, and autophagy in transplanted tumors of mouse models also confirmed the results in HCC cell lines. Finally, we find that HULC acts as an endogenous sponge, which abolishes the binding of miR-107 on the Atg12 3 '-UTR and promotes autophagy and metastasis of HCC.
基金：
National Scientific and Technological Projects for Major Infectious Diseases [2017ZX10203205003-004]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81700556]; National Major Scientific and Technological Special Project for Significant New Drugs Development [2014ZX09101005004]; Key Scientific and Technological Project ofHenan Province [202102310126]
第一作者单位：[1]Liver Transplantation Center, National Clinical Research Center for Digestive Diseases and Beijing Key Laboratory of Tolerance Induction and Organ Protection in Transplantation, Beijing Friendship Hospital, Capital Medical University, Beijing, China
共同第一作者：
通讯作者：
通讯机构：[1]Liver Transplantation Center, National Clinical Research Center for Digestive Diseases and Beijing Key Laboratory of Tolerance Induction and Organ Protection in Transplantation, Beijing Friendship Hospital, Capital Medical University, Beijing, China[3]Department of Immunology, Tianjin Key Laboratory of Cellular and Molecular Immunology, Tianjin Medical University, Tianjin, China[*1]Liver TransplantationCenter, National ClinicalResearch Center for DigestiveDiseases, Beijing Friendship Hospital,Capital Medical University, Beijing 100050,China.[*2]Department of Immunology, TianjinKey Laboratory ofCellular and Molecular Immunology,Tianjin Medical University,Tianjin, 300070,China.
推荐引用方式(GB/T 7714)：
Haiming Zhang,Shipeng Li,Haixu Xu,et al.Interference of miR-107 with Atg12 is inhibited by HULC to promote metastasis of hepatocellular carcinoma[J].MEDCOMM.2020,1(2):165-177.doi:10.1002/mco2.25.
APA：
Haiming Zhang,Shipeng Li,Haixu Xu,Liying Sun,Zhijun Zhu&Zhi Yao.(2020).Interference of miR-107 with Atg12 is inhibited by HULC to promote metastasis of hepatocellular carcinoma.MEDCOMM,1,(2)
MLA：
Haiming Zhang,et al."Interference of miR-107 with Atg12 is inhibited by HULC to promote metastasis of hepatocellular carcinoma".MEDCOMM 1..2(2020):165-177
医学