单位：[1]Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, Northern China, China[2]Nuclear Medicine Department, Beijing Friendship Hospital, Capital Medical University, Beijing, Northern China, China医技科室影像中心核医学科首都医科大学附属北京友谊医院
Neuroblastoma (NB) is an embryonic malignant tumour of the sympathetic nervous system, and current research shows that activation of brown adipose tissue accelerates cachexia in cancer patients. However, the interaction between brown adipose tissues and NB remains unclear. The study aimed to investigate the effect of brown adipocytes in the co-culture system on the proliferation and migration of NB cells. Brown adipocytes promoted the proliferation and migration of Neuro-2a, BE(2)-M17, and SH-SY5Y cells under the co-culture system, with an increase of the mRNA and protein levels of UCP2 and PPAR-gamma in NB cells. The UCP2 inhibitor genipin or PPAR-gamma inhibitor T0090709 inhibited the migration of NB cells induced by brown adipocytes. Genipin or siUCP2 upregulated the expression of E-cadherin, and downregulated the expression of N-cadherin and vimentin in NB cells. We suggest that under co-cultivation conditions, NB cells can activate brown adipocytes, which triggers changes in various genes and promotes the proliferation and migration of NB cells. The PPAR-gamma/UCP2 pathway is involved in the migration of NB cells caused by brown adipocytes.
基金：
Beijing Natural Science Foundation [7192041, 7202132]; CAMS Innovation Fund for Medical Sciences (CIFMS) [2021-I2M -1-030]
第一作者单位：[1]Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, Northern China, China
通讯作者：
通讯机构：[1]Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, Northern China, China[2]Nuclear Medicine Department, Beijing Friendship Hospital, Capital Medical University, Beijing, Northern China, China[*1]Nuclear Medicine Department, Beijing Friendship Hospital, Capital Medical University, Beijing, Northern China, China[*2]Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, Northern China, China
推荐引用方式(GB/T 7714)：
Ge Zhijuan,Shang Yue,Wang Wendie,et al.Brown adipocytes promote epithelial mesenchymal transition of neuroblastoma cells by inducing PPAR-gamma/UCP2 expression[J].ADIPOCYTE.2022,11(1):335-345.doi:10.1080/21623945.2022.2073804.
APA：
Ge Zhijuan,Shang Yue,Wang Wendie,Yang Jigang&Chen Shu-Zhen.(2022).Brown adipocytes promote epithelial mesenchymal transition of neuroblastoma cells by inducing PPAR-gamma/UCP2 expression.ADIPOCYTE,11,(1)
MLA：
Ge Zhijuan,et al."Brown adipocytes promote epithelial mesenchymal transition of neuroblastoma cells by inducing PPAR-gamma/UCP2 expression".ADIPOCYTE 11..1(2022):335-345
