单位：[1]Department of Nephrology, Beijing Friendship Hospital, Faculty of Kidney Diseases, Capital MedicalUniversity, Beijing, China临床科室肾脏内科肾脏内科首都医科大学附属北京友谊医院[2]Center of Allergy, Beijing Shijitan Hospital, Capital Medical University, Beijing, China
OBJECTIVE: In recent years, the death number of renal cell carcinoma (RCC) has been enhanced annually. The crucial function of long non-coding RNA (lncRNA) in the occurrence and progression of cancer is of great significance. However, the specific role of lncRNAs in the pathogenesis and prognosis of RCC has not been fully elucidated. Therefore, the aim of this study was to uncover the role of lncRNA RP11-567G11.1 in regulating the progression of RCC. PATIENTS AND METHODS: Relative expression level of RP11-567G11.1 in RCC tissues and cells was determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The influences of RP11-567G11.1 on proliferative and invasive abilities of RCC cells were assessed. Subsequently, regulatory effects of RP11-567G11.1 on the viability and apoptosis of DDP-induced RCC cells were examined. Furthermore, the mRNA and protein levels of Notch pathway-related genes Jagged1/HES5/HEY1 in RCC were detected by qRT-PCR and Western blot, respectively. RESULTS: RP11-567G11.1 expression was significantly up-regulated in RCC tissues and cells. Meanwhile, RP11-567G11.1 was highly expressed in RCC patients with advanced stage. Knockdown of RP11-567G11.1 significantly attenuated proliferative and invasive abilities of 786-O and 769-P cells. Silence of RP11-567G11.1 attenuated viability, while it induced apoptosis in DDP-induced RCC cells. In addition, knockdown of RP11-567G11.1 remarkably down-regulated both mRNA and protein levels of Jagged1, HES5, and HEY1 in RCC. CONCLUSIONS: RP11-567G11.1 accelerates the proliferative and invasive abilities of RCC through activating the Notch pathway. Our findings suggest that it may be a new therapeutic target for RCC.
基金：
Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support [ZYLX201824]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81570660]; Beijing Shijitan Hospital Funding [2016-C02, 2017-C02]
第一作者单位：[1]Department of Nephrology, Beijing Friendship Hospital, Faculty of Kidney Diseases, Capital MedicalUniversity, Beijing, China
通讯作者：
通讯机构：[1]Department of Nephrology, Beijing Friendship Hospital, Faculty of Kidney Diseases, Capital MedicalUniversity, Beijing, China[2]Center of Allergy, Beijing Shijitan Hospital, Capital Medical University, Beijing, China
推荐引用方式(GB/T 7714)：
Liu S.,Liu W-H,Diao Z-L,et al.LncRNA RP11-567G11.1 accelerates the proliferation and invasion of renal cell carcinoma through activating the Notch pathway[J].EUROPEAN REVIEW for MEDICAL and PHARMACOLOGICAL SCIENCES.2020,24(9):4738-4744.
APA：
Liu, S.,Liu, W-H,Diao, Z-L,Zhang, A-H,Guo, W....&Huang, H-D.(2020).LncRNA RP11-567G11.1 accelerates the proliferation and invasion of renal cell carcinoma through activating the Notch pathway.EUROPEAN REVIEW for MEDICAL and PHARMACOLOGICAL SCIENCES,24,(9)
MLA：
Liu, S.,et al."LncRNA RP11-567G11.1 accelerates the proliferation and invasion of renal cell carcinoma through activating the Notch pathway".EUROPEAN REVIEW for MEDICAL and PHARMACOLOGICAL SCIENCES 24..9(2020):4738-4744
