单位：[1]College of Animal Science and Technology, Institute of Mobilome and Genome, Yangzhou University, Yangzhou, China[2]Institute of Basic Medical Sciences, Peking Union Medical College–Chinese Academy of Medical Sciences, Beijing, China[3]Guangdong Key Laboratory for Genome Stability and Disease Prevention, Shenzhen University School of Medicine, Shenzhen, China[4]Department of Andrology, China-Japan Friendship Hospital, Beijing, China
Mumps virus (MuV) has high tropism to the testis and may lead to male infertility. Sertoli cells are the major targets of MuV infection. However, the mechanisms by which MuV infection impairs male fertility and Sertoli cell function remain unclear. The present study elucidated the effect of MuV infection on the blood-testis barrier (BTB). The transepithelial electrical resistance of MuV-infected mouse Sertoli cells was monitored, and the expression of major proteins of the BTB was examined. We demonstrated that MuV infection disrupted the BTB by reducing the levels of occludin and zonula occludens 1. Sertoli cells derived from Tlr2(-/-) and Tnfa(-/-) mice were analyzed for mediating MuV-induced impairment. TLR2-mediated TNF-alpha production by Sertoli cells in response to MuV infection impaired BTB integrity. MuV-impaired BTB was not observed in Tlr2(-/-) and Tnfa(-/-) Sertoli cells. Moreover, an inhibitor of TNF-alpha, pomalidomide, prevents the disruption of BTB in response to MuV infection. FITC-labeled biotin tracing assay confirmed that BTB permeability and spermatogenesis were transiently impaired by MuV infection in vivo. These findings suggest that the disruption of the BTB could be one of the mechanisms underlying MuV-impaired male fertility, in which TNF-alpha could play a critical role.
基金：
Chinese Academy of Medical Sciences (CAMS) Initiative for Innovative Medicine [2017-IZM-BR-06, 2017-IZM-3-007]; Major State Basic Research Project of China [2016YFA0101001, 2018YFC1003902]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [1701430]; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2017M611931]
第一作者单位：[1]College of Animal Science and Technology, Institute of Mobilome and Genome, Yangzhou University, Yangzhou, China[2]Institute of Basic Medical Sciences, Peking Union Medical College–Chinese Academy of Medical Sciences, Beijing, China
共同第一作者：
通讯作者：
通讯机构：[1]College of Animal Science and Technology, Institute of Mobilome and Genome, Yangzhou University, Yangzhou, China[2]Institute of Basic Medical Sciences, Peking Union Medical College–Chinese Academy of Medical Sciences, Beijing, China[*1]College of Animal Science and Technology, Institute of Mobilome and Genome, Yangzhou University, 88 Daxue South Rd., Yangzhou 225009, China.[*2]Department of Cell Biology, Institute of Basic Medical Sciences, Peking Union Medical College–Chinese Academy of Medical Sciences, 5 Dong Dan San Tiao, Beijing 100005, China.
推荐引用方式(GB/T 7714)：
Wu Han,Jiang Xing,Gao Yunxiao,et al.Mumps virus infection disrupts blood-testis barrier through the induction of TNF-alpha in Sertoli cells[J].FASEB JOURNAL.2019,33(11):12528-12540.doi:10.1096/fj.201901089R.
APA：
Wu, Han,Jiang, Xing,Gao, Yunxiao,Liu, Weihua,Wang, Fei...&Han, Daishu.(2019).Mumps virus infection disrupts blood-testis barrier through the induction of TNF-alpha in Sertoli cells.FASEB JOURNAL,33,(11)
MLA：
Wu, Han,et al."Mumps virus infection disrupts blood-testis barrier through the induction of TNF-alpha in Sertoli cells".FASEB JOURNAL 33..11(2019):12528-12540
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