单位：[1]Institute of Dermatology and Department of Dermatology, the First Affiliated Hospital, Anhui Medical University, Hefei, Anhui, China[2]Department of Dermatology, China-Japan Friendship Hospital, Beijing, China[3]Institute of Dermatology and Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China
The polymorphism of PRKCB has been proven to be associated with systemic lupus erythematosus (SLE) in our previous study. We aimed to investigate the relationship between expression of PRKCB mRNA and the Disease Activity Index (SLEDAI) and manifestations of SLE. Quantitative reverse transcription polymerase chain reaction (RT-PCR) was applied to examine the expression of PRKCB mRNA in peripheral blood mononuclear cells of 60 patients with SLE and 62 controls. The Sequenom MassArray System was used to detect genotype SNP rs16972959. The expression levels of PRKCB mRNA in SLE cases were significantly increased compared with those in healthy controls (P<0.001). In addition, PRKCB mRNA expression levels were negatively correlated with the SLEDAI (P<0.05, r=-0.322), with lower mRNA expression levels of PRKCB in patients found with higher SLEDAI, presence of a new rash (P<0.01), and proteinuria (P<0.05). No association evidence was observed between the genotype of the variant rs16972959 and PRKCB mRNA expression levels; however, SNP rs16972959 was found to be an expression quantitative trait loci for PRKCB with the SLE risk allele correlated with increased expression in naive monocytes (P-FDR=9.12x10(-13)) and stimulated monocytes (9.24x10(-6)>P-FDR>2.75x10(-17)). On the other hand, SNP rs16972959 of PRKCB was found to have suggestive significant associations with vasculitis (P=0.00718) of SLE. These results indicated that expression of PRKCB mRNA may be correlated with the pathogenesis of SLE; however, more investigation is still needed.
基金：
National Key Research and Development Program of China [2017YFC0909000, 2017YFC0909001]; National Key Basic Research Program of ChinaNational Basic Research Program of China [2014CB541901, 2011CB512103]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81602397, 81573033, 81402590, 81371722, 81320108016, 81171505]; Research Project of Chinese Ministry of EducationMinistry of Education, China [213018A]; Program for New Century Excellent Talents in UniversityProgram for New Century Excellent Talents in University (NCET) [NCET-12-0600]; Natural Science Foundation of Anhui ProvinceNatural Science Foundation of Anhui Province [1408085MKL27]; Natural Science Foundation of ShanghaiNatural Science Foundation of Shanghai [15ZR1405700]
第一作者单位：[1]Institute of Dermatology and Department of Dermatology, the First Affiliated Hospital, Anhui Medical University, Hefei, Anhui, China
共同第一作者：
通讯作者：
通讯机构：[1]Institute of Dermatology and Department of Dermatology, the First Affiliated Hospital, Anhui Medical University, Hefei, Anhui, China[2]Department of Dermatology, China-Japan Friendship Hospital, Beijing, China[*1]Department of Dermatology,China- Japan FriendshipHospital,Yinghua street, Chaoyang District, Beijing 100029, China[*2]Institute of Dermatology and Department of Dermatology, the FirstAffiliated Hospital, Anhui MedicalUniversity 81 MeishanRoad, ShushanDistrict, Hefei, Anhui 230032, China.
推荐引用方式(GB/T 7714)：
Zhengwei Zhu,Lulu Yang,Yaohua Zhang,et al.Increased expression of PRKCB mRNA in peripheral blood mononuclear cells from patients with systemic lupus erythematosus[J].ANNALS of HUMAN GENETICS.2018,82(4):200-205.doi:10.1111/ahg.12240.
APA：
Zhengwei Zhu,Lulu Yang,Yaohua Zhang,Lu Liu,Yan Huang...&Yong Cui.(2018).Increased expression of PRKCB mRNA in peripheral blood mononuclear cells from patients with systemic lupus erythematosus.ANNALS of HUMAN GENETICS,82,(4)
MLA：
Zhengwei Zhu,et al."Increased expression of PRKCB mRNA in peripheral blood mononuclear cells from patients with systemic lupus erythematosus".ANNALS of HUMAN GENETICS 82..4(2018):200-205
