单位：[1]Department of Otolaryngology—Head and Neck Surgery, The First Affiliated Hospital of China Medical University, Shenyang, China[2]Department of Otolaryngology, Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China临床科室耳鼻咽喉头颈外科耳鼻咽喉头颈外科首都医科大学附属北京友谊医院[3]Department of Otolaryngology, The No 463rd Hospital of PLA, Shenyang, China[4]Department of Otolaryngology—Head and Neck Surgery, The First Affiliated Hospital of DaLian Medical Universty, Dalian, China大连医科大学附属第一医院[5]Department of Neurosurgery, Medical Faculty of the Friedrich Alexander University of Erlangen-Numberg (FAU), Erlangen, Germany[6]Department of Otolaryngology—Head and Neck Surgery, General Hospital of PLA and the E.N.T Institute of PLA, Beijing, China
Background: Previous study showed that mild ototoxic exposure could induce a reversible hearing impairment, and the loss and secondary incomplete recovery of cochlear ribbon synapses could be responsible for the hearing loss. However, it remains unclear whether cochlear outer hair cells' (OHCs) functions are affected. Objective: To verify whether the function of OHCs are also affected significantly after the ototoxic exposure. Methods: Mice were injected intraperitoneally with 100mg/kg concentration of gentamicin daily for 14 days. Distortion Product of Oto-acoustic Emission (DPOAE) was detected at control (pre-treatment), Day 0, day 4, day 7, day 14 and day 28 after the ototoxic exposure, respectively. In addition, the morphology of OHCs was observed by electron microscopy, OHCs has been counted by light microscopy, and the hearing thresholds were detected by auditory brain response (ABR). Results: No significant changes have been found in OHC and OHC stereocilia among the experimental groups (p > .05). Further, no significant changes or loss was found in the morphology of OHCs either. However, we found ABR threshold elevations occurred after ototoxic exposure. Conclusions: Unitary ototoxic gentamicin exposure may not disrupt the function of cochlear OHCs in mice, regardless of hearing loss identified in this ototoxic exposure.
基金：
National High-Tech R&D Program of China for Young; Beijing Municipal Commission of EducationBeijing Municipal Commission of Education [TJSHG 201510025004]; National Natural Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [81503372, 81271720]; Youth Cultivation Project of Military Medical Science [16QNP133]
第一作者单位：[1]Department of Otolaryngology—Head and Neck Surgery, The First Affiliated Hospital of China Medical University, Shenyang, China[2]Department of Otolaryngology, Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China
共同第一作者：
通讯作者：
通讯机构：[2]Department of Otolaryngology, Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China[*1]Department of Otolaryngology Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China
推荐引用方式(GB/T 7714)：
Zhao Ning,Tai XuHui,Zhai LiJie,et al.Unitary ototoxic gentamicin exposure may not disrupt the function of cochlear outer hair cells in mice[J].ACTA OTO-LARYNGOLOGICA.2017,137(8):842-849.doi:10.1080/00016489.2017.1295470.
APA：
Zhao, Ning,Tai, XuHui,Zhai, LiJie,Shi, Lin,Chen, DaiShi...&Liu, Ke.(2017).Unitary ototoxic gentamicin exposure may not disrupt the function of cochlear outer hair cells in mice.ACTA OTO-LARYNGOLOGICA,137,(8)
MLA：
Zhao, Ning,et al."Unitary ototoxic gentamicin exposure may not disrupt the function of cochlear outer hair cells in mice".ACTA OTO-LARYNGOLOGICA 137..8(2017):842-849
