单位：[a]The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Beijing, China[b]Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, China[c]School of Biomedical Sciences and Shenzhen Institute of Research and Innovation, The University of Hong Kong, Pokfulam, Hong Kong[d]The Centre for Synthetic Biology Engineering Research, Shenzhen Institutes of Advanced Technology, Shenzhen, China
Insulin stimulates adiponectin secretion and glucose transporter type 4 (GLUT4) translocation in adipocyte to regulate metabolism homeostasis. Similar to GLUT4 translocation, intracellular trafficking and release of adiponectin in adipocytes relies on the trans-Golgi network and endosomal system. Recent studies show that the heavy chain of conventional kinesin (KIF5B) mediates GLUT4 translocation in murine 3T3-L1 adipocytes, however, the motor machinery involved in mediating intracellular trafficking and release of adiponectin is unknown. Here, we examined the role of KIF5B in the regulation of adiponectin secretion. The KIF5B level was up-regulated during 3T3-L1 adipogenesis. This increase in cytosolic KIF5B was synchronized with the induction of adiponectin. Endogenous KIF5B and adiponectin were partially colocalized at the peri-nuclear and cytosolic regions. In addition, adiponectin-containing vesicles were co-immunoprecipitated with KIF5B. Knockdown of KIF5B resulted in a marked inhibition of adiponectin secretion and overexpression of KIF5B enhanced adiponectin release, whereas leptin secretion was not affected by changes in KIF5B expression. These data suggest that the secretion of adiponectin, but not leptin, is dependent on functional KIF5B. (C) 2016 Elsevier Inc. All rights reserved.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31400995]; Beijing Natural Science FoundationBeijing Natural Science Foundation [7154234]; National Science and Technology Project [2012BAI10B01]; Hong Kong Research Grants CouncilHong Kong Research Grants Council [HKU 767012, HKU 768113M, HKU 17127015, HKUST10/CRF/12R]
第一作者单位：[a]The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Beijing, China[*1]The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Beijing, China.
通讯作者：
通讯机构：[a]The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Beijing, China[c]School of Biomedical Sciences and Shenzhen Institute of Research and Innovation, The University of Hong Kong, Pokfulam, Hong Kong[*1]The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Beijing, China.[*2]School of Biomedical Sciences and Shenzhen Institute of Research and Innovation, The University of Hong Kong, Pokfulam, Hong Kong
推荐引用方式(GB/T 7714)：
Ju Cui,Jing Pang,Ya-Jun Lin,et al.Conventional kinesin KIF5B mediates adiponectin secretion in 3T3-L1 adipocytes[J].BIOCHEMICAL and BIOPHYSICAL RESEARCH COMMUNICATIONS.2016,476(4):620-626.doi:10.1016/j.bbrc.2016.06.008.
APA：
Ju Cui,Jing Pang,Ya-Jun Lin,Ping Jiang,Huan Gong...&Tie-Mei Zhang.(2016).Conventional kinesin KIF5B mediates adiponectin secretion in 3T3-L1 adipocytes.BIOCHEMICAL and BIOPHYSICAL RESEARCH COMMUNICATIONS,476,(4)
MLA：
Ju Cui,et al."Conventional kinesin KIF5B mediates adiponectin secretion in 3T3-L1 adipocytes".BIOCHEMICAL and BIOPHYSICAL RESEARCH COMMUNICATIONS 476..4(2016):620-626
