单位：[1]Beijing Hosp, Key Lab Geriatr, Beijing, Peoples R China[2]Beijing Inst Geriatr, Minist Hlth, Beijing, Peoples R China[3]Univ Hong Kong, LKS Fac Med, Dept Biochem, Hong Kong, Hong Kong, Peoples R China[4]China Japan Friendship Hosp, Inst Clin Med Sci, Beijing, Peoples R China[5]Shenzhen Inst Adv Technol, Ctr Synthet Biol Engn Res, Shenzhen, Peoples R China
Polarization of epithelial cells requires vectorial sorting and transport of polarity proteins to apical or basolateral domains. Kif5b is the mouse homologue of the human ubiquitous Kinesin Heavy Chain (uKHC). To investigate the function of Kif5b in epithelial cells, we examined the phenotypes of Kif5b-deficient MDCK cells. Stable knockdown of Kif5b in MDCK cells resulted in reduced cell proliferation rate, profound changes in cell morphology, loss of epithelial cell marker, and gain of mesenchymal marker, as well as increased cell migration, invasion, and tumorigenesis abilities. E-cadherin and NMMIIA could interact with Kif5b in polarized MDCK cells, and their expression levels were decreased in Kif5b-deficient MDCK cells. Overexpression of E-cadherin and NMMIIA in Kif5b depleted MDCK cells could decrease mesenchymal marker expression and cell migration ability. These results indicate that stable knockdown of Kif5b in MDCK cells can lead to epithelial-mesenchymal transition, which is mediated by defective E-cadherin and NMMIIA expression. (C) 2015 Elsevier Inc. All rights reserved.
基金：
Hong Kong Research Grants CouncilHong Kong Research Grants Council [HKU 768113M, HKU 767012]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31400995]; Beijing Municipal Natural Science FoundationBeijing Natural Science Foundation [7154234]
第一作者单位：[1]Beijing Hosp, Key Lab Geriatr, Beijing, Peoples R China[2]Beijing Inst Geriatr, Minist Hlth, Beijing, Peoples R China[3]Univ Hong Kong, LKS Fac Med, Dept Biochem, Hong Kong, Hong Kong, Peoples R China
通讯作者：
通讯机构：[1]Beijing Hosp, Key Lab Geriatr, Beijing, Peoples R China[2]Beijing Inst Geriatr, Minist Hlth, Beijing, Peoples R China[3]Univ Hong Kong, LKS Fac Med, Dept Biochem, Hong Kong, Hong Kong, Peoples R China
推荐引用方式(GB/T 7714)：
Cui Ju,Jin Guoxiang,Yu Bin,et al.Stable knockdown of Kif5b in MDCK cells leads to epithelial-mesenchymal transition[J].BIOCHEMICAL and BIOPHYSICAL RESEARCH COMMUNICATIONS.2015,463(1-2):123-129.doi:10.1016/j.bbrc.2015.05.045.
APA：
Cui, Ju,Jin, Guoxiang,Yu, Bin,Wang, Zai,Lin, Raozhou&Huang, Jian-Dong.(2015).Stable knockdown of Kif5b in MDCK cells leads to epithelial-mesenchymal transition.BIOCHEMICAL and BIOPHYSICAL RESEARCH COMMUNICATIONS,463,(1-2)
MLA：
Cui, Ju,et al."Stable knockdown of Kif5b in MDCK cells leads to epithelial-mesenchymal transition".BIOCHEMICAL and BIOPHYSICAL RESEARCH COMMUNICATIONS 463..1-2(2015):123-129
