单位：[1]Univ Hong Kong, Li Ka Shing Fac Med, Dept Biochem, Pokfulam, Hong Kong, Peoples R China[2]China Japan Friendship Hosp, Inst Clin Med Sci, Beijing, Peoples R China[3]Minist Hlth, Beijing Hosp, Beijing Inst Geriatr, Beijing, Peoples R China
The microtubule motor kinesin-1 is responsible for the nuclear positioning during myogenesis. Here we show that the coiled-coil stalk/tail domain containing the kinesin light chain (KLC) binding sites targets to the perinuclear region like endogenous Kif5b, while the globular tail domain cannot. To investigate which fragments of kinesin heavy chain (Kif5b) is responsible for the myonuclear positioning, we transfect Kif5b expression constructs into Kif5b deficient myoblasts and test their ability to rescue the myonuclear phenotype. We find that the KLC binding domain and the autoinhibitory peptide in the globular tail region are both indispensable for the nuclear membrane localization of Kif5b and the kinesin-1-mediated myonuclear positioning. These results suggest that while the KLC binding domain may directly targets Kif5b to the myonuclear membrane, the autoinhibitory peptide may play an indirect role in regulating the kinesin-l-mediated myonuclear positioning. (C) 2013 Elsevier Inc. All rights reserved.
基金：
Hong Kong Research Grants CouncilHong Kong Research Grants Council [HKU 767110M, HKU 767012]; Hong Kong University Seed Funding Programme for Basic Research [200911159033]
第一作者单位：[1]Univ Hong Kong, Li Ka Shing Fac Med, Dept Biochem, Pokfulam, Hong Kong, Peoples R China[2]China Japan Friendship Hosp, Inst Clin Med Sci, Beijing, Peoples R China
通讯作者：
通讯机构：[1]Univ Hong Kong, Li Ka Shing Fac Med, Dept Biochem, Pokfulam, Hong Kong, Peoples R China[2]China Japan Friendship Hosp, Inst Clin Med Sci, Beijing, Peoples R China
推荐引用方式(GB/T 7714)：
Wang Zai,Xue Wenqian,Li Xiuling,et al.Dissect Kif5b in nuclear positioning during myogenesis: The light chain binding domain and the autoinhibitory peptide are both indispensable[J].BIOCHEMICAL and BIOPHYSICAL RESEARCH COMMUNICATIONS.2013,432(2):242-247.doi:10.1016/j.bbrc.2013.02.006.
APA：
Wang, Zai,Xue, Wenqian,Li, Xiuling,Lin, Raozhou,Cui, Ju&Huang, Jian-Dong.(2013).Dissect Kif5b in nuclear positioning during myogenesis: The light chain binding domain and the autoinhibitory peptide are both indispensable.BIOCHEMICAL and BIOPHYSICAL RESEARCH COMMUNICATIONS,432,(2)
MLA：
Wang, Zai,et al."Dissect Kif5b in nuclear positioning during myogenesis: The light chain binding domain and the autoinhibitory peptide are both indispensable".BIOCHEMICAL and BIOPHYSICAL RESEARCH COMMUNICATIONS 432..2(2013):242-247
