单位：[1]School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029, China[2]Department of Pharmacology, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, China[3]Department of Pharmacy, China-Japan Friendship Hospital, Beijing 100029, China[4]Classical Prescription Application Foundation Research Innovation Team, Beijing University of Chinese Medicine, Beijing 100029, China
Theacrine (1,3,7,9-tetramethyluric acid), a purine alkaloid similar to caffeine in its chemical structure, is isolated from edible Camellia assamica var. kucha and has various pharmacological activities including hypnotic effects, anti-depressant effects, anti-inflammatory and analgesic effects, and a protective effect against stress-provoked liver damage. A rapid and simple assay is required to quantify theacrine in biological samples for pharmacokinetic studies in small animals. This study aimed to establish an enzyme-linked immunosorbent assay (ELISA) for theacrine quantification in blood. Herein, we successfully obtained monoclonal antibodies (MAbs) against theacrine, MAbs C11135, and developed an ELISA method for the fast determination of theacrine in mouse blood. The range for calibration of theacrine by ELISA was 0.156-100 mu g mL(-1). The half maximum inhibitory concentration (IC50) value was 1.55 mu g mL(-1). The ELISA method lays a good foundation for the further research.(C) 2016 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81473590, 81274115, 81473119]; China Japan Friendship Hospital Youth Science and Technology Excellence Project [2014-QNYC-B-10]; Program for New Century Excellent Talents in University of the Chinese Ministry of Education [NCET-11-0606]; Program for Excellent Talents of Beijing Municipal Party Committee Organization Department of the Communist Party of China [2013D009999000001]
第一作者单位：[1]School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029, China[2]Department of Pharmacology, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, China
通讯作者：
通讯机构：[1]School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029, China[4]Classical Prescription Application Foundation Research Innovation Team, Beijing University of Chinese Medicine, Beijing 100029, China[*1]School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029, China.
推荐引用方式(GB/T 7714)：
Li Zhuang,He Jun,Zhang Yue,et al.Development of an enzyme-linked immunosorbent assay using specific monoclonal antibodies against theacrine and its application[J].CHINESE CHEMICAL LETTERS.2016,27(12):1771-1775.doi:10.1016/j.cclet.2016.06.052.
APA：
Li, Zhuang,He, Jun,Zhang, Yue,Wang, Qing Guo,Zhao, Yan...&Xu, Jie Kun.(2016).Development of an enzyme-linked immunosorbent assay using specific monoclonal antibodies against theacrine and its application.CHINESE CHEMICAL LETTERS,27,(12)
MLA：
Li, Zhuang,et al."Development of an enzyme-linked immunosorbent assay using specific monoclonal antibodies against theacrine and its application".CHINESE CHEMICAL LETTERS 27..12(2016):1771-1775
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