The noggin gene is present in the central nervous system in embryonic and postnatal mammals, and plays an important role in maintaining nervous system development and physiological function. A 0.76-kb sequence of human noggin gene was cloned by polymerase chain reaction with the digestion site of Hind III and Xba I on the 5' end. The cloned fragment was reversely inserted into pCS2+[T alpha 1]-GFP plasmid, an neural cell-specific antisense eukaryotic expression vector. The plasmid expresses antisense for human noggin specifically in neurons, which may facilitate understanding of the physiological function of noggin.
基金：
Science and Technology Development Program of Shandong Province [2007GG10002017]; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [Y2008C63]
第一作者单位：[1]Shandong Univ, Dept Neurol, Qilu Hosp, Jinan 250012, Shandong, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Zhou Shengnian,Li Chengshan,Wei Xiansen,et al.Construction of neuron specific vector of human antisense noggin gene expression[J].NEURAL REGENERATION RESEARCH.2010,5(15):1171-1174.doi:10.3969/j.issn.1673-5374.2010.15.009.
APA：
Zhou, Shengnian,Li, Chengshan,Wei, Xiansen,Liu, Liqing&Zhang, Zhengda.(2010).Construction of neuron specific vector of human antisense noggin gene expression.NEURAL REGENERATION RESEARCH,5,(15)
MLA：
Zhou, Shengnian,et al."Construction of neuron specific vector of human antisense noggin gene expression".NEURAL REGENERATION RESEARCH 5..15(2010):1171-1174
