Hybrid capture II (HC-II) is the only technique that can be used in clinical human papillomavirus (HPV) DNA detection However, there is controversy in regards to how to analyze and assess the viral load of high-risk (FIR)-HPV by dse of HC-II and the relation between viral load and cervical lesions In this study, we analyzed the results oi a sequential screening of outpatients at the Department of Obstetrics and Gynecology of the China-Japan Friendship Hospital, and we aimed to explore the relationship between HR-HPV viral load i and the severity of cervical lesions, and to clarify the clinical significance of the titer of HR-HPV DNA determined by HC-II Using HC-II, 2,761 women were screened for HR-HPV DNA clombined with cytological testing using liquid-based cytology All women with HR-HPV-positive results or abnormalities in cytology underwent a cervical biopsy through colposcopy Cervical biopsies were taken in 1,051 women he HR-HPV infection rate was 78 35% (76/97) in HPV-associated lesions, 87 33% (193/221) in cervical intraepithelial neoplasia (CIN) I, 94 74% (144/152) in CIN H, 100% (178/178) in CIN III and 160% (20/20) in invasive cervical cancer (ICC), respectively (P<0 05) Based on the criteria of histopathology, the sensitivity Of HR-HPV DNA testing by HC-H for detecting high-grade 1 cervical lesions was 9771%, the specificity was 79 64%, the positive-predictive value was 41 06% and the negativejiredictive value was 9959% The viral loads of HR-HPV DNA were 512 15 +/- 764 19 in HPV-associated lesions, 753 95 +/- 978 27 in CIN I, 871 08 +/- 1003 52 in CIN II, 603 40 740 25 in CIN III and 466 44 +/- 673 05 in ICC, respectively In conclusion, the positive rate of HR-HPV increased significantly in accordance with the severity of cervical lesions The viral loads of cervical inflammatory lesions were markedly lower than CINs and ICC The viral loads of HR-HPV DNA tested by HC-II had no correlation with the grade of cervical lesions