Numerous studies have confirmed that oligodendrocyte transcription factor 1 (Olig-1) is vital for myelin repair. However, the effects of hypoxia and ischemia on Olig-1 expression remain unknown. In this study, Olig-1 mRNA and protein expressions were analyzed by in situ hybridization and immunohistochemistry, to determine the expression profile of Olig-1 in rat brain slices exposed to hypoxia and ischemia. Brains were obtained from 2-day-old Sprague-Dawley rats, and sections were randomly assigned to control and hypoxia/ischemia groups. Hematoxylin-eosin staining revealed karyorrhexis and karyopyknosis in cells from the hypoxia/ischemia group. Under electron microscopy, mitochondria swelling and neuropil edema were observed in the hypoxia/ischemia group. Olig-1 mRNA and protein expressions were increased at 1 day after hypoxia and ischemia treatment. These results suggest that in situ hybridization and immunohistochemistry could be used simultaneously to detect mRNA and protein expression in brain slices.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30872778]; Natural Science Foundation of Beijing CityBeijing Natural Science Foundation [7072023]; Clinical Basic Corporation Foundation of Capital Medical University [2006jl18]
第一作者单位：[1]Capital Med Univ, Dept Pediat, Beijing Friendship Hosp, Beijing 100050, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Cui Hong,Yang Lijun,Huang Dezhuang,et al.Oligodendrocyte transcription factor 1 mRNA and protein expression in organotypic rat brain slices[J].NEURAL REGENERATION RESEARCH.2010,5(21):1639-1643.doi:10.3969/j.issn.1673-5374.2010.21.007.
APA：
Cui, Hong,Yang, Lijun,Huang, Dezhuang,Zhang, Wandong,Han, Weijuan...&Jiang, Wenxing.(2010).Oligodendrocyte transcription factor 1 mRNA and protein expression in organotypic rat brain slices.NEURAL REGENERATION RESEARCH,5,(21)
MLA：
Cui, Hong,et al."Oligodendrocyte transcription factor 1 mRNA and protein expression in organotypic rat brain slices".NEURAL REGENERATION RESEARCH 5..21(2010):1639-1643
