单位：[1]China Japan Friendship Hosp, Dept Dermatol, Beijing 100029, Peoples R China[2]Beijing Med Univ, First Hosp, Dept Dermatol, Beijing 100034, Peoples R China[3]Beijing Normal Univ, Dept Biol, State Key Lab Cell Proliferat & Regulat Biol, Beijing 100875, Peoples R China
Protein kinase G (PKC), which has a wide variety of functions, comprises at least twelve isoenzymes that have distinct roles respectively. Research in recent years indicates that PKC zeta plays a pivotal role in the regulation of cell proliferation. In order to investigate its role in the regulation of keratinocyte proliferation, eukaryotic expression plasmid of anti-sense PKC zeta is constructed and transfected into human keratinocyte line, Colo16. The human keratinocyte model expressing anti-sense PKC zeta is established successfully, the growth properties of the cell model is analyzed by the detection of cell growth curve and cell cycle phase. The mechanism of the relationship between PKC zeta and CyclinD1 is also investigated by the western blotting analysis of CyclinD1 protein.
第一作者单位：[1]China Japan Friendship Hosp, Dept Dermatol, Beijing 100029, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Zhang XY,Liu HT,Ma SQ.Construction and primary analysis of human keratinocyte model expressing anti-sense protein kinase C zeta[J].CHINESE SCIENCE BULLETIN.1998,43(18):1556-1562.doi:10.1007/BF02883448.
APA：
Zhang, XY,Liu, HT&Ma, SQ.(1998).Construction and primary analysis of human keratinocyte model expressing anti-sense protein kinase C zeta.CHINESE SCIENCE BULLETIN,43,(18)
MLA：
Zhang, XY,et al."Construction and primary analysis of human keratinocyte model expressing anti-sense protein kinase C zeta".CHINESE SCIENCE BULLETIN 43..18(1998):1556-1562
