The tcdA-negative and tcdB-positive Clostridium difficile ST81 clone exhibits a high level of resistance to fluoroquinolones: a multi-centre study in Beijing, China
单位：[1]Center of Clinical Laboratory, Beijing Friendship Hospital, Capital Medical University, Beijing, China医技科室检验科检验科首都医科大学附属北京友谊医院[2]Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China[3]Department of Clinical Laboratory, Beijing Huaxin Hospital, the First Affiliated[4]Charles Sturt University, Leeds Parade, Orange, New South Wales, Australia[5]Centre for Infectious Diseases and Microbiology Laboratory Services, Westmead Hospital, Westmead, New South Wales, Australia[6]Department of Infectious Diseases and Clinical Microbiology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China北京朝阳医院[7]Department of Clinical Laboratory, Aerospace Center Hospital, Beijing, China[8]Department of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, Capital Medical University, Beijing, China
Clostridium difficile infection (CDI) is the leading cause of antibiotic-associated diarrhoea worldwide. In order to gain a better understanding about the molecular epidemiology of C. difficile in Beijing, China, molecular typing, antimicrobial susceptibility testing and drug resistance gene sequencing were performed on 174 strains of C. difficile collected from four large tertiary hospitals in Beijing. In total, 31 sequence types (STs) were identified among the 174 strains. ST81 was found to be the most prevalent (26.4%, 46/174), followed by ST2 (16.7%, 29/174) and ST54 (9.8%, 17/174). All isolates were susceptible to metronidazole and vancomycin. The test strains displayed resistance rates of 97.1%, 44.3% and 44.3% for ciprofloxacin, levofloxacin and moxifloxacin, respectively. ST81 isolates displayed a drug resistance rate of 97.8% for levofloxacin and moxifloxacin, which was significantly higher than ST2 (0%), ST54 (17.6%) and ST42 (0%) isolates (P<0.05). An amino acid mutation (T82I) was identified in GyrA, and the total mutation rate of the C. difficile strains was 40.8% (71/174). The mutation rate of ST81 isolates was 95.7% (44/46). Three amino acid mutations (D426N, S366A and D426V) were identified in GyrB, and the total mutation rate of GyrB was 39.1%. A double-site mutation in GyrB (S366A+D426V) was identified in all ST81 (n=46) isolates. In conclusion, the C. difficile ST81 clone showed a high level of resistance to fluoroquinolones in Beijing, highlighting the need for nationwide surveillance of CDI. (c) 2020 Published by Elsevier B.V.
基金：
Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81501807]; National Key Research and Development Program of China [2017YFC1601502]
第一作者单位：[1]Center of Clinical Laboratory, Beijing Friendship Hospital, Capital Medical University, Beijing, China[2]Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China
通讯作者：
推荐引用方式(GB/T 7714)：
Cheng J.-W,Liu C,Kudinha T,et al.The tcdA-negative and tcdB-positive Clostridium difficile ST81 clone exhibits a high level of resistance to fluoroquinolones: a multi-centre study in Beijing, China[J].INTERNATIONAL JOURNAL of ANTIMICROBIAL AGENTS.2020,56(1):doi:10.1016/j.ijantimicag.2020.105981.
APA：
Cheng, J.-W,Liu, C,Kudinha, T,Xiao, M,Fan, X...&Xu, Y.-C.(2020).The tcdA-negative and tcdB-positive Clostridium difficile ST81 clone exhibits a high level of resistance to fluoroquinolones: a multi-centre study in Beijing, China.INTERNATIONAL JOURNAL of ANTIMICROBIAL AGENTS,56,(1)
MLA：
Cheng, J.-W,et al."The tcdA-negative and tcdB-positive Clostridium difficile ST81 clone exhibits a high level of resistance to fluoroquinolones: a multi-centre study in Beijing, China".INTERNATIONAL JOURNAL of ANTIMICROBIAL AGENTS 56..1(2020)
