Background: Endoplasmic reticulum stress (ERS) plays an important role in the process of myocardial hypertrophy in diabetic cardiomyopathy (DCM). Irisin, a novel cytokine, has been found to protect against cardiac diastolic dysfunction in DCM. We aimed to investigate the role of irisin in cardiac hypertrophy and to elucidate the underlying mechanisms.Methods: H9c2 cells were induced with 33 mM glucose to construct a cardiac hypertrophy cell model, which was then treated with irisin in the presence or absence of the ERS inducer tunicamycin (TM). The cell surface area was measured by FITC-phalloidin staining. The atrial natriuretic peptide levels were detected by an enzyme linked immunosorbent assay. Furthermore, the expression of the ERS-related proteins, P-PERK, PERK, IRE1 alpha and GRP78, was detected by western blotting.Results: Irisin significantly reduced myocardial hypertrophy and suppressed high glucose (HG)-induced oxidative stress. Meanwhile, the protective effect of irisin on cardiomyoblasts was reversed by the ERS inducer, TM. Additionally, we detected ERS-associated signaling pathway proteins and found that irisin significantly reduced the protein expression levels of GRP78 and p-PERK/PERK.Conclusion: These results suggest that irisin ameliorates HG-induced cardiac hypertrophy by inhibiting ERS.
基金：
Natural Science Foundation of Inner Mongolia Autonomous Region [2020MS08174, 2018MS08045]; Scientific Research Project of Higher Education Institutions in Inner Mongolia Autonomous Region [NJZZ20189, RDY2021-28]; Peking University People's Hospital Scientific Research Development Funds; Hainan Province Clinical Medical Center
第一作者单位：[1]Chifeng Univ, Sch Clin Med, Chifeng, Inner Mongolia, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[8]Chifeng Univ, Sch Stomatol, Chifeng, Inner Mongolia, Peoples R China[*1]Chifeng Univ, Sch Stomatol, Chifeng 024000, Inner Mongolia, Peoples R China
推荐引用方式(GB/T 7714)：
Li Xiujun,Zhang Da-Qi,Wang Xiaohui,et al.Irisin alleviates high glucose-induced hypertrophy in H9c2 cardiomyoblasts by inhibiting endoplasmic reticulum stress[J].PEPTIDES.2022,152:doi:10.1016/j.peptides.2022.170774.
APA：
Li, Xiujun,Zhang, Da-Qi,Wang, Xiaohui,Zhang, Qin,Qian, Liu...&Li, Xiuli.(2022).Irisin alleviates high glucose-induced hypertrophy in H9c2 cardiomyoblasts by inhibiting endoplasmic reticulum stress.PEPTIDES,152,
MLA：
Li, Xiujun,et al."Irisin alleviates high glucose-induced hypertrophy in H9c2 cardiomyoblasts by inhibiting endoplasmic reticulum stress".PEPTIDES 152.(2022)
