Vaccination With a Single Consensus Envelope Protein Ectodomain Sequence Administered in a Heterologous Regimen Induces Tetravalent Immune Responses and Protection Against Dengue Viruses in Mice
单位：[1]Department of Microbiology and Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, China, [2]Beijing Tropical Medicine Research Institute, Beijing Friendship Hospital, The Second Clinical Medical College of Capital Medical University, Beijing, China首都医科大学附属北京友谊医院[3]Viral Disease and Vaccine Translational Research Unit, CAS Key Laboratory of Molecular Virology and Immunology, Institute Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China[4]Center of Epilepsy, Beijing Institute for Brain Disorders, Beijing, China
The development of a safe and effective tetravalent dengue vaccine that elicits protection against all dengue virus (DENV) serotypes is urgently needed. The consensus sequence of the ectodomain of envelope (E) protein of DENV (cE80) has been examined as an immunogen previously. In the current study, a cE80 DNA (D) vaccine was constructed and evaluated in conjunction with the cE80 protein (P) vaccine to examine whether both vaccines used together can further improve the immune responses. The cE80 DNA vaccine was administrated using either a homologous (DNA alone, DDD) or heterologous (DNA prime-protein boost: DDP or DPP) regimen, and evaluated for immunogenicity and protective efficacy in mice. Among the three DNA-based immunization regimens tested, DDP immunization is the optimal immunization regimen that elicited the greatest systemic immune response and conferred protection against all four DENV serotypes. This work provides innovative ideas for the development of consensus E-based dengue vaccines and the testing of optimal immunization regimens.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81671971, 81471957, 81772172, 81372935, 31370870, 31670941]; Scientific Research Plan of the Beijing Municipal Education Committee [KZ201810025035]; National Key R&D Program of China [2016YFC1201000]; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDB29040301]
第一作者单位：[1]Department of Microbiology and Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, China,
通讯作者：
通讯机构：[1]Department of Microbiology and Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, China, [4]Center of Epilepsy, Beijing Institute for Brain Disorders, Beijing, China
推荐引用方式(GB/T 7714)：
Wang Ran,Zheng Xiaoyan,Sun Jin,et al.Vaccination With a Single Consensus Envelope Protein Ectodomain Sequence Administered in a Heterologous Regimen Induces Tetravalent Immune Responses and Protection Against Dengue Viruses in Mice[J].FRONTIERS in MICROBIOLOGY.2019,10:doi:10.3389/fmicb.2019.01113.
APA：
Wang, Ran,Zheng, Xiaoyan,Sun, Jin,Feng, Kaihao,Gao, Na...&An, Jing.(2019).Vaccination With a Single Consensus Envelope Protein Ectodomain Sequence Administered in a Heterologous Regimen Induces Tetravalent Immune Responses and Protection Against Dengue Viruses in Mice.FRONTIERS in MICROBIOLOGY,10,
MLA：
Wang, Ran,et al."Vaccination With a Single Consensus Envelope Protein Ectodomain Sequence Administered in a Heterologous Regimen Induces Tetravalent Immune Responses and Protection Against Dengue Viruses in Mice".FRONTIERS in MICROBIOLOGY 10.(2019)
