单位：[1]Department of Microbiology and Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, China,[2]Beijing Tropical Medicine Research Institute, Beijing Friendship Hospital, Capital Medical University, Beijing, China,首都医科大学附属北京友谊医院[3]YunnanProvincial Key Laboratory of Vector-borne Disease Control and Research, Yunnan Institute of Parasitic Diseases, Pu’er,China,[4]Center of Epilepsy, Beijing Institute for Brain Disorders, Beijing, China
Dengue virus (DENV) is the causative agent of dengue, and its incidence has increased 30-fold in the past five decades. Among the four cocirculating serotypes, DENV3 is associated with an increased number of severe infections and has become widespread. Vaccination is the mainstay of prevention in reducing disease burden. Previously, the protective efficacy of DNA vaccine candidates toward DENV1, 2, and 4 was confirmed in mice. In this study, a DNA vaccine candidate (pVAX1-D3ME) expressing the prM and E proteins of DENV3 was constructed, and then the immunogenicity and protection were assessed in mice to further develop a tetravalent dengue vaccine. Moreover, the cross-reactive immune responses against the other three serotypes were investigated. The results showed that three doses of 50 mu g of pVAX1-D3ME were sufficient to induce strong antigen-specific T cell responses and robust and consistent neutralizing antibodies. Additionally, immunization with pVAX1-D3ME offered protective immunity against not only DENV3 but also the other three serotypes, which could be observed even after 12 months. This study shows great promise for the further evaluation of a dengue tetravalent DNA vaccine candidate in large animal models, including non-human primates.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81772172, 81671971, U1602223, 81372935]; Beijing Municipal Commission of EducationBeijing Municipal Commission of Education [KZ201810025035]
第一作者单位：[1]Department of Microbiology and Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, China,
通讯作者：
通讯机构：[1]Department of Microbiology and Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, China,[4]Center of Epilepsy, Beijing Institute for Brain Disorders, Beijing, China
推荐引用方式(GB/T 7714)：
Feng Kaihao,Zheng Xiaoyan,Wang Ran,et al.Long-Term Protection Elicited by a DNA Vaccine Candidate Expressing the prM-E Antigen of Dengue Virus Serotype 3 in Mice[J].FRONTIERS in CELLULAR and INFECTION MICROBIOLOGY.2020,10:doi:10.3389/fcimb.2020.00087.
APA：
Feng, Kaihao,Zheng, Xiaoyan,Wang, Ran,Gao, Na,Fan, Dongying...&An, Jing.(2020).Long-Term Protection Elicited by a DNA Vaccine Candidate Expressing the prM-E Antigen of Dengue Virus Serotype 3 in Mice.FRONTIERS in CELLULAR and INFECTION MICROBIOLOGY,10,
MLA：
Feng, Kaihao,et al."Long-Term Protection Elicited by a DNA Vaccine Candidate Expressing the prM-E Antigen of Dengue Virus Serotype 3 in Mice".FRONTIERS in CELLULAR and INFECTION MICROBIOLOGY 10.(2020)
