单位：[1]Capital Univ Med Sci, Beijing Friendship Hosp Affiliate, Dept Neurol, Beijing 100050, Peoples R China临床科室神经内科神经内科首都医科大学附属北京友谊医院[2]Beijing Med Univ, United Biomed Ctr, Beijing 100083, Peoples R China
In this study, DNA vaccines consisting of vector IRES-gD expressing Herpes simplex virus 1 (HSV-1) glycoprotein D (gD) and vector IRES-gD-IL-2 coexpressing HSV-1 gD and interleukin-2 (IL-2), respectively, were constructed. After intramuscular inoculation, both vaccines induced in BALB/c mice antibodies as assayed by ELISA and virus neutralization. However, IRES-gD-IL-2 elicited significantly higher levels of IgG (ELISA) and neutralizing antibodies than IRES-gD. Isotyping of sera from mice injected with IRES-gD-IL-2 revealed predominately IgG2a antibodies. IRES-gD-IL-2 also elicited a higher delayed-type sensitivity (DTH) reaction. However, there was no difference in the protection against lethal challenge with HSV-1 between the two vaccines (P > 0.05). The results suggest that the vaccination with IRES-gD-IL-2 can efficiently enhance the immune response of mice to HSV-1, particularly through increased cellular immunity.
第一作者单位：[1]Capital Univ Med Sci, Beijing Friendship Hosp Affiliate, Dept Neurol, Beijing 100050, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Li W. -R.,Niu B.,Wang J. -W.,et al.Coexpression of interleukin-2 enhances the immunization effect of a DNA vaccine expressing herpes simplex 1 glycoprotein D[J].ACTA VIROLOGICA.2006,50(4):251-256.
APA：
Li, W. -R.,Niu, B.,Wang, J. -W.,Feng, Z. -J.&Wang, D. -X..(2006).Coexpression of interleukin-2 enhances the immunization effect of a DNA vaccine expressing herpes simplex 1 glycoprotein D.ACTA VIROLOGICA,50,(4)
MLA：
Li, W. -R.,et al."Coexpression of interleukin-2 enhances the immunization effect of a DNA vaccine expressing herpes simplex 1 glycoprotein D".ACTA VIROLOGICA 50..4(2006):251-256
