单位：[1]Department of Otolaryngology-Head and Neck Surgery, Guangdong Second Provincial General Hospital, Southern Medical University, Guangzhou, People’s Republic of China[2]Department of Otolaryngology-Head and Neck Surgery, Chinese PLA General Hospital, Beijing, People’s Republic of China[3]Department of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, People’s Republic of China临床科室耳鼻咽喉头颈外科耳鼻咽喉头颈外科首都医科大学附属北京友谊医院[4]Department of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital of China Medical University, Shenyang, People’s Republic of China[5]Department of Otolaryngology-Head and Neck Surgery, Guangdong Second Provincial General Hospital, Guangzhou, People's Republic of China
Objective: To investigate whether there are more quantitative pre-synaptic ribbons formed in the cochlear region corresponding to middle-frequency in cochlea of mice. Methods: Counts of pre-synaptic ribbons were performed using immunostaining and laser confocal microscopy. Hearing thresholds and function of ribbon synapses were estimated by auditory brain response (ABR) and compound action potential (CAP). Cochlear mapping has been achieved to match the frequencies and corresponding regions along the cochlear spiral. Results: The number of pre-synaptic ribbons in per inner hair cell (IHC) has been found to increase gradually from the base turn, the maximal quantity appeared at the region of 50-70% from the apex. Next, ABR thresholds showed that there was the lowest ABR threshold in the frequency around 8-16 kHz, corresponding to the region of 50-70% from the apex according to the cochlear mapping. Further, CAP amplitudes were estimated, and the maximal value identified at the same frequency (8-16 kHz). Conclusions: Maximal number of pre-synaptic ribbons is formed in the cochlear region of middle frequency in mice, coupling with the lowest ABR threshold and highest CAP amplitudes. Our study shows that the middle frequency (8-16 kHz) could be the most sensitive region to sound stimuli in mice.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31040038]; Beijing Natural Science FoundationBeijing Natural Science Foundation [5122040]; National Basic Research Program of China (973Program)National Basic Research Program of China [2012CB967900]; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [201003779, 20100470103]
第一作者单位：[1]Department of Otolaryngology-Head and Neck Surgery, Guangdong Second Provincial General Hospital, Southern Medical University, Guangzhou, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[3]Department of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, People’s Republic of China[5]Department of Otolaryngology-Head and Neck Surgery, Guangdong Second Provincial General Hospital, Guangzhou, People's Republic of China[*1]Department of Otolaryngology-Head and Neck Surgery, Chinese PLA General Hospital, Beijing, People’s Republic of China[*2]Department of Otolaryngology-Head and Neck Surgery, Guangdong Second Provincial General Hospital, Guangzhou, People’s Republic of China
推荐引用方式(GB/T 7714)：
Yang Le,Chen DaiShi,Qu TengFei,et al.Maximal number of pre-synaptic ribbons are formed in cochlear region corresponding to middle frequency in mice[J].ACTA OTO-LARYNGOLOGICA.2018,138(1):25-30.doi:10.1080/00016489.2017.1367417.
APA：
Yang, Le,Chen, DaiShi,Qu, TengFei,Ding, TongHui,Yan, AiHui...&Liu, Ke.(2018).Maximal number of pre-synaptic ribbons are formed in cochlear region corresponding to middle frequency in mice.ACTA OTO-LARYNGOLOGICA,138,(1)
MLA：
Yang, Le,et al."Maximal number of pre-synaptic ribbons are formed in cochlear region corresponding to middle frequency in mice".ACTA OTO-LARYNGOLOGICA 138..1(2018):25-30
