单位：[1]Department of Neurology, Beijing Friendship Hospital, Capital Medical University, Yongan Rd 95, Xicheng District, Beijing 100050, People’s Republic of China临床科室神经内科神经内科首都医科大学附属北京友谊医院[2]State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou, Zhejiang, China[3]National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Chang-Bai Rd 155, Beijing 102206, People’s Republic of China[4]Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Galectin-1 (Gal-1) shows neuroprotective activity in brain ischemia, spinal cord injury, and autoimmune neuroinflammation. To evaluate the Gal-1 situation in the brains of prion disease, the brain levels of Gal-1 in several scrapie-infected experimental rodent models were tested by Western blot, including agents 263K-infected hamsters, 139A-, ME7-, and S15-infected mice. Remarkable increases of brain Gal-1 were observed in all tested scrapie-infected rodents at the terminal stage. The brain levels of Gal-1 showed time-dependent increases along with the prolonging of incubation times. Immunohistochemical assays illustrated much stronger stainings in the brain sections of scrapie-infected rodents. Quantitative RT-PCR of Gal-1 gene demonstrated increased transcription in the brains of scrapie-infected mice. Gal-1 was colocalized with GFAP- and NeuN-positive cells, but not with Iba-1-positive cells in immunofluorescent test. Increases of Gal-1 were also detected in the several postmortem cortex regions of human prion diseases. Moreover, the S-nitrosylated forms of Gal-1 in the brains of scrapie-infected rodents were significantly higher than those of normal ones. Our finding here demonstrates markedly increased brain Gal-1 and S-nitrosylated Gal-1 both in scrapie-infected rodents and human prion diseases.
基金：
Chinese National Natural Science FoundationNational Natural Science Foundation of China (NSFC) [81301032, 81301429, 81572048]; China Mega-Project for Infectious Disease [2011ZX10004-101, 2012ZX10004215]; SKLID Development Grant [2012SKLID102, 2015SKLID503]
第一作者单位：[1]Department of Neurology, Beijing Friendship Hospital, Capital Medical University, Yongan Rd 95, Xicheng District, Beijing 100050, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[2]State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou, Zhejiang, China[3]National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Chang-Bai Rd 155, Beijing 102206, People’s Republic of China[4]Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
推荐引用方式(GB/T 7714)：
Guo Yan-Jun,Shi Qi,Yang Xiao-Dong,et al.Increases of Galectin-1 and its S-nitrosylated form in the Brain Tissues of Scrapie-Infected Rodent Models and Human Prion Diseases[J].MOLECULAR NEUROBIOLOGY.2017,54(5):3707-3716.doi:10.1007/s12035-016-9923-1.
APA：
Guo, Yan-Jun,Shi, Qi,Yang, Xiao-Dong,Li, Jian-Le,Ma, Yue...&Dong, Xiao-Ping.(2017).Increases of Galectin-1 and its S-nitrosylated form in the Brain Tissues of Scrapie-Infected Rodent Models and Human Prion Diseases.MOLECULAR NEUROBIOLOGY,54,(5)
MLA：
Guo, Yan-Jun,et al."Increases of Galectin-1 and its S-nitrosylated form in the Brain Tissues of Scrapie-Infected Rodent Models and Human Prion Diseases".MOLECULAR NEUROBIOLOGY 54..5(2017):3707-3716
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