单位：[1]Key laboratory of Photochemistry, Institute of Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100190, China[2]Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100190, China[3]Dearpment of Ednocrinology, China-Japan Friendship Hospital, Beijing 100029, China[4]Dearpment of laboratory Medicine, China-Japan Friendship Hospital, Beijing 100029, China
Abnormal expression of gamma-Glutamyltranspeptidase (GGT) in living organisms is closely implicated in the development of several human tumors. The GGT levels in tissue and serum have emerged as a potential criterion for tumor diagnosis. However, precise "light up" GGT activity in vivo is still challenging due to the signal interferes of background. Bioluminescence based on the firefly luciferase-catalyzed reaction for light production provides a feasible strategy for GGT detection in vivo. In this report, a bioluminogenic probe, Glu-Luc, was designed and synthesized by connecting D-luciferin with gamma-glutamyl group. The cleavage of gamma-glutamyl group is triggered by GGT, resulting in the release of D-luciferin, which generates a bright bioluminescence emission in the present of luciferase and ATP. The probe exhibits very high selectivity and sensitivity toward GGT activity from in vitro to in vivo and in clinical samples, which offers a promising tool for investigations of the GGT-overexpressing related biological process including tumor diagnosis and prognosis in living organisms.
基金：
973 ProgramNational Basic Research Program of China [2013CB834505, 2013CB834703]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21233011, 91123033, 21273252, 21205122, 21261160488]
第一作者单位：[1]Key laboratory of Photochemistry, Institute of Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100190, China
通讯作者：
推荐引用方式(GB/T 7714)：
Li Shuang,Hu Rui,Yang Chenlin,et al.An ultrasensitive bioluminogenic probe of gamma-Glutamyltranspeptidase in vivo and in human serum for tumor diagnosis[J].BIOSENSORS & BIOELECTRONICS.2017,98:325-329.doi:10.1016/j.bios.2017.06.059.
APA：
Li, Shuang,Hu, Rui,Yang, Chenlin,Zhang, Xin,Zeng, Yi...&Yang, Guoqiang.(2017).An ultrasensitive bioluminogenic probe of gamma-Glutamyltranspeptidase in vivo and in human serum for tumor diagnosis.BIOSENSORS & BIOELECTRONICS,98,
MLA：
Li, Shuang,et al."An ultrasensitive bioluminogenic probe of gamma-Glutamyltranspeptidase in vivo and in human serum for tumor diagnosis".BIOSENSORS & BIOELECTRONICS 98.(2017):325-329
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