单位：[1]Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China[2]Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China[3]University of the Chinese Academy of Sciences, Beijing 100049, China[4]Beijing Key Lab for Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, China[5]Qingdao Municipal Hospital, Qingdao, China
We investigated the renal protective effects of low molecular weight fucoidan (LMWF) and its two fractions (F0.5 and F1.0), which were extracted from Laminaria japonica, on the epithelial-mesenchymal transition (EMT) induced by transforming growth factor beta 1 (TGF-beta 1) and fibroblast growth factor 2 (FGF-2) in HK-2 human renal proximal tubular cells. Cell morphology and EMT markers (fibronectin and alpha-smooth muscle actin) demonstrated that cells treated with TGF-beta 1 or FGF-2 developed EMT to a significant extent. Treatment with LMWF or its fractions markedly attenuated the EMT and decreased expression of the EMT markers. The F1.0 fraction, the sulfated fucan fraction, was found to be the main active component of LMWF, and heparanase (HPSE) was a key factor in renal tubular epithelial trans differentiation. The F1.0 fraction inhibited elevated HPSE and matrix metallopeptidase 9 expression, thereby attenuating the progress of EMT. (C) 2017 Elsevier B.V. All rights reserved.
基金：
Natural Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [41376166, 41406144, 81620108031, 81473526]; Project of Discovery, Evaluation and Transformation of Active Natural Compounds, Strategic Biological Resources Service Network Program of Chinese Academy of Sciences [ZSZH-0025]; Key Research and Development Project of Shandong Province [2016YYSP002, 2016GSF115031]; Youth Innovation Promotion Association of CAS [2016190]
第一作者单位：[1]Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China[2]Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China[3]University of the Chinese Academy of Sciences, Beijing 100049, China
通讯作者：
通讯机构：[1]Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China[2]Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China[4]Beijing Key Lab for Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, China[*1]Institute of Oceanology, Chinese Academy of Sciences,Qingdao 266071, China.
推荐引用方式(GB/T 7714)：
Li Xinpeng,Li Xiaohong,Zhang Quanbin,et al.Low molecular weight fucoidan and its fractions inhibit renal epithelial mesenchymal transition induced by TGF-beta 1 or FGF-2[J].INTERNATIONAL JOURNAL of BIOLOGICAL MACROMOLECULES.2017,105:1482-1490.doi:10.1016/j.ijbiomac.2017.06.058.
APA：
Li, Xinpeng,Li, Xiaohong,Zhang, Quanbin&Zhao, Tingting.(2017).Low molecular weight fucoidan and its fractions inhibit renal epithelial mesenchymal transition induced by TGF-beta 1 or FGF-2.INTERNATIONAL JOURNAL of BIOLOGICAL MACROMOLECULES,105,
MLA：
Li, Xinpeng,et al."Low molecular weight fucoidan and its fractions inhibit renal epithelial mesenchymal transition induced by TGF-beta 1 or FGF-2".INTERNATIONAL JOURNAL of BIOLOGICAL MACROMOLECULES 105.(2017):1482-1490
生物学