单位：[1]Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China临床科室国家中心普外分中心普外五科（综合普外科）首都医科大学附属北京友谊医院[2]Beijing Key Laboratory of Cancer Invasion and Metastasis Research & National Clinical Research Center of Digestive Diseases, Beijing 100050, Chinaa临床科室肿瘤中心首都医科大学附属北京友谊医院
In this study, chitosan/heparin immobilized delivery system was developed for the delivery of sorafenib in gastric cancers. The SRF NP was nanosized with spherical outfit and present in the amorphous form. The SRF NP exhibited a sustained release of drug at pH 7.4 conditions and enhanced drug released at pH 5.5 conditions. Flow cytometer analysis showed that cellular uptake of NP increased two-fold after 4 h of incubation compared to 1 h incubation. The SRF NP showed superior anticancer effect compared to that of free SRF in BGC-823 cancer cells. SRF NP induced a remarkable apoptosis of cancer cells consistent with the cytotoxicity assay. Approximately, 50% of cell fractions were observed in early apoptosis phase with similar to 15% of cells in the late apoptosis stage. Consistently, SRF NP exhibited a strong band for caspase-3 and P-53 than compared to free SRF in MGC-823 cancer cells. Importantly, SRF NP showed superior anticancer effect in xenograft tumor model making it a promising delivery vehicle in the treatment of gastric cancers. (C) 2015 Elsevier Ltd. All rights reserved.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81172317, 30972887]; Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support [ZYLX201504]
第一作者单位：[1]Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China[2]Beijing Key Laboratory of Cancer Invasion and Metastasis Research & National Clinical Research Center of Digestive Diseases, Beijing 100050, Chinaa
通讯作者：
通讯机构：[1]Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China[2]Beijing Key Laboratory of Cancer Invasion and Metastasis Research & National Clinical Research Center of Digestive Diseases, Beijing 100050, Chinaa[*1]Department of General Surgery, Beijing FriendshipHospital, Capital Medical University, No. 95, Yongan Road, Xicheng District, 100050Beijing, China
推荐引用方式(GB/T 7714)：
Yang YingChi,Cai Jun,Yin Jie,et al.Heparin-functionalized Pluronic nanoparticles to enhance the antitumor efficacy of sorafenib in gastric cancers[J].CARBOHYDRATE POLYMERS.2016,136:782-790.doi:10.1016/j.carbpol.2015.09.023.
APA：
Yang, YingChi,Cai, Jun,Yin, Jie,Zhang, Jun,Wang, KangLi&Zhang, ZhongTao.(2016).Heparin-functionalized Pluronic nanoparticles to enhance the antitumor efficacy of sorafenib in gastric cancers.CARBOHYDRATE POLYMERS,136,
MLA：
Yang, YingChi,et al."Heparin-functionalized Pluronic nanoparticles to enhance the antitumor efficacy of sorafenib in gastric cancers".CARBOHYDRATE POLYMERS 136.(2016):782-790
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