单位：[1]Fac Med, CNRS, UMR 7563 LEMTA, F-54500 Vandoeuvre Les Nancy, France[2]China Japan Friendship Hosp, Res Inst Clin Med Sci, Beijing 100029, Peoples R China[*1]Fac Med, CNRS, UMR 7563 LEMTA, BP 184, F-54500 Vandoeuvre Les Nancy, France
The aim of this work was to study the influences of Ca2+ (medium free calcium, with BAPTA, with 100 mM Ca2+, 100 mM Ca2++10 mu M ionomycin) on the expression of caveolin-1 (structural protein of caveolae) of endothelial cells (ECs) submitted to mechanical forces (shear stress) or biochemical stimulation (TNF-alpha). We found that shear stress enhanced the caveolin-1 expression. Simultaneously, the caveolin-1 expression is dependant on [Ca2+]i: [Ca2+](free medium+100 mu M BAPTA)<[Ca2+](free medium)<[Ca2+](100 mM)<[Ca2+](100 mM+10 mu M ionomycin). In contrast, TNF-alpha induced a decrease of caveolin-1 in the cells, whatever the [Ca2+]i. These results suggest that there could be a synergistic effect between shear stress and Ca2+ on caveolin-1 expression but an inflammatory stimulation (TNF-alpha) induces a down regulation of caveolin-1 expression.
通讯机构：[*1]Fac Med, CNRS, UMR 7563 LEMTA, BP 184, F-54500 Vandoeuvre Les Nancy, France
推荐引用方式(GB/T 7714)：
Fawzi-Grancher S,Sun RJ,Traore M,et al.Role of Ca2+ in the effects of shear stress and TNF-alpha on caveolin-1 expression[J].CLINICAL HEMORHEOLOGY and MICROCIRCULATION.2005,33(3):253-261.
APA：
Fawzi-Grancher, S,Sun, RJ,Traore, M,Stoltz, JF&Muller, S.(2005).Role of Ca2+ in the effects of shear stress and TNF-alpha on caveolin-1 expression.CLINICAL HEMORHEOLOGY and MICROCIRCULATION,33,(3)
MLA：
Fawzi-Grancher, S,et al."Role of Ca2+ in the effects of shear stress and TNF-alpha on caveolin-1 expression".CLINICAL HEMORHEOLOGY and MICROCIRCULATION 33..3(2005):253-261
