单位：[1]Peking Univ, Coll Life Sci, Natl Lab Biomembrane & Membrane Biotechnol, Beijing 100871, Peoples R China[2]China Japan Friendship Hosp, Inst Clin Med Sci, Beijing 100029, Peoples R China
The actions of the opioid agonist U50488H on I-A and I-K were examined in acutely isolated mouse hippocampal CA3 pyramidal neurons using the whole-cell patch clamp technique. U50488H caused a concentration dependent, rapidly developing and reversible inhibition of voltage-activated I-A and I-K. The inhibitory actions were still observed in the presence of 30 muM naloxone or 5 muM nor-binaltorphimine dihydrochloride. The IC50 values for the blockade of I-A and I-K were calculated as 20.1.9 and 3.7 muM, respectively. In the presence of 3.3 muM U50488H, repetitive stimulation induced use-dependent inhibition of I-A and I-K. A 10 muM concentration of U50488H positively shifted the half-activation membrane potential of I-A by +11 mV, but negatively shifted I-K by -14 mV. These results demonstrate that U50488H can directly inhibit neuronal I-A and I-K without involvement of the activation of kappa -opioid receptors. (C) 2001 Elsevier Science B.V. All rights reserved.
Zou BD,OuYang KF,Chen YZ,et al.Blockade of U50488H on potassium currents of acutely isolated mouse hippocampal CA3 pyramidal neurons[J].BRAIN RESEARCH.2001,897(1-2):52-59.doi:10.1016/S0006-8993(00)03330-8.
APA：
Zou, BD,OuYang, KF,Chen, YZ,Wu, CH&Zhou, PA.(2001).Blockade of U50488H on potassium currents of acutely isolated mouse hippocampal CA3 pyramidal neurons.BRAIN RESEARCH,897,(1-2)
MLA：
Zou, BD,et al."Blockade of U50488H on potassium currents of acutely isolated mouse hippocampal CA3 pyramidal neurons".BRAIN RESEARCH 897..1-2(2001):52-59
