Dichloromethane Extract of Fermentation Broth by Co-Culture of Morchella esculenta and Coprinus comatus Induces Apoptosis in U251 Cells via Mitochondrial Intrinsic Pathway
单位：[1]Dalian University, Liaoning 116622, P.R. China[2]Department of Engineering Physics, Institute of Medical Physics, Tsinghua University, Beijing 100084, P.R. China[3]College of Life Science, Dezhou University, Shandong 253023, P.R. China[4]Division of Medical Oncology, Department of Integrated Traditional and Western Medicine, China-Japan Friendship Hospital, Beijing 100029, P.R. China
The current study was designed to investigate the inhibitory effects of dichloromethane extract of fermentation broth by co-culture of Motthella esculenta and Coprinus comatus (DCMM) on human glioma U251 cells in vitro and its possible underlying mechanisms. The proliferation of U251 cells was inhibited by DCMM with different concentrations by the CCK-8 assay. Besides, flow cytometry assay was used to evaluate the DCMM promoted U251 cell apoptosis rate in a dose-dependent manner. DCMM with different concentrations (10 mu g. mL(-1), 20 mu g. mL(-1), and 40 mu g. mL(-1)) significantly enhanced the expression of caspases-3 activity after 24 h. In addition, DCMM with different concentrations significantly increased caspase-3 and Bax, and decreased Bcl-2 expressions at both mRNA and protein levels. DCMM can remarkably inhibit the proliferation and promote cell apoptosis of human glioma U251 cells. The possible underlying mechanisms could be related to induction of apoptosis of human glioma U251 cells by mitochondrial intrinsic pathway.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21402104, 81570188]
第一作者单位：[1]Dalian University, Liaoning 116622, P.R. China
通讯作者：
通讯机构：[1]Dalian University, Liaoning 116622, P.R. China[2]Department of Engineering Physics, Institute of Medical Physics, Tsinghua University, Beijing 100084, P.R. China[*1]Dalian University, Liaoning 116622, P.R. China[*2]Department of Engineering Physics, Institute of Medical Physics, Tsinghua University, Beijing 100084, P.R. China
推荐引用方式(GB/T 7714)：
Zhong Xue,Li Qian,Wang Huiguo,et al.Dichloromethane Extract of Fermentation Broth by Co-Culture of Morchella esculenta and Coprinus comatus Induces Apoptosis in U251 Cells via Mitochondrial Intrinsic Pathway[J].INTERNATIONAL JOURNAL of MEDICINAL MUSHROOMS.2020,22(10):1001-1010.doi:10.1615/IntJMedMushrooms.2020036163.
APA：
Zhong, Xue,Li, Qian,Wang, Huiguo,Zhao, Xiaohong,Yu, Hongzhu...&Tang, Jintian.(2020).Dichloromethane Extract of Fermentation Broth by Co-Culture of Morchella esculenta and Coprinus comatus Induces Apoptosis in U251 Cells via Mitochondrial Intrinsic Pathway.INTERNATIONAL JOURNAL of MEDICINAL MUSHROOMS,22,(10)
MLA：
Zhong, Xue,et al."Dichloromethane Extract of Fermentation Broth by Co-Culture of Morchella esculenta and Coprinus comatus Induces Apoptosis in U251 Cells via Mitochondrial Intrinsic Pathway".INTERNATIONAL JOURNAL of MEDICINAL MUSHROOMS 22..10(2020):1001-1010
