Overexpression of apoptosis-inducing factor mitochondrion-associated 1 (AIFM1) induces apoptosis by promoting the transcription of caspase3 and DRAM in hepatoma cells
单位：[a]Beijing Institute of Hepatology, Beijing, 100069, China[b]Capital Medical University Affiliated Beijing You an Hospital, Beijing, 100069, China[c]Beijing Precision Medicine and Transformation Engineering Technology Research Center of Hepatitis and Liver Cancer, 100069, China[d]Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014040, China内蒙古科技大学包头医学院[e]General Hospital of Heilongjiang Province Land Reclamation Bureau, Harbin 150088, China[f]Beijing Friendship Hospital, Capital Medical University, 100050, China首都医科大学附属北京友谊医院
Full-length apoptosis-inducing factor mitochondrion-associated 1 (AIFM1) (similar to 67 kDa) induces apoptosis in a caspase-independent manner when it is cleaved at its N-terminus to produce truncated AIFM1 (similar to 57 kDa). Here, we produced recombinant adenovirus AIFM1 (rAd-AIFM1) encoding full-length AIFM1 to detect whether full-length AIFM1 suppresses cell growth and induces apoptosis of hepatoma cell lines (HepG2 and Hep3B). Hepatocellular carcinoma (HCC) is one of the most difficult cancers to treat worldwide. The MIT assay demonstrated that full-length AIFM1 inhibited the growth of hepatoma cells because rAd-AIFM1 infection suppressed the proliferation of HepG2 and Hep3B cells. TUNEL assay demonstrated that full-length AIFM1 overexpression induced apoptosis in HepG2 and Hep3B cells infected with rAd-AIFM1, suggesting an apoptosis-inducing ability of full-length AIFM1. Our data further showed that the expression of two pro-apoptotic genes, caspase3 and DRAM, were involved in fulllength AIFM1 infection-induced apoptosis, and full-length AIFM1 could also positively regulate the transcription of caspase3 and DRAM. Thus, overexpression of full-length AIFM1 can induce caspase-dependent apoptosis and suppresses cell growth of hepatoma cells. Our data uncover a potential role of rAd-AIFM1 in HCC gene therapy. (C) 2018 Elsevier Inc. All rights reserved.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [NSFC 81402556, 81773168]; Foundation of Beijing Institute of Hepatology [BJIH-01715]
第一作者单位：[a]Beijing Institute of Hepatology, Beijing, 100069, China[b]Capital Medical University Affiliated Beijing You an Hospital, Beijing, 100069, China[c]Beijing Precision Medicine and Transformation Engineering Technology Research Center of Hepatitis and Liver Cancer, 100069, China[d]Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014040, China
共同第一作者：
通讯作者：
通讯机构：[a]Beijing Institute of Hepatology, Beijing, 100069, China[b]Capital Medical University Affiliated Beijing You an Hospital, Beijing, 100069, China[c]Beijing Precision Medicine and Transformation Engineering Technology Research Center of Hepatitis and Liver Cancer, 100069, China[*1]No. 8 XiTouTiao, YouAnMenWai Street, FengTai District, Beijing 100069, China.[*2]No. 8 XiTouTiao, YouAnMenWai Street, FengTai District, Beijing 100069, China.
推荐引用方式(GB/T 7714)：
Dongjie Liu,Mingyang Liu,Wenjing Wang,et al.Overexpression of apoptosis-inducing factor mitochondrion-associated 1 (AIFM1) induces apoptosis by promoting the transcription of caspase3 and DRAM in hepatoma cells[J].BIOCHEMICAL and BIOPHYSICAL RESEARCH COMMUNICATIONS.2018,498(3):453-457.doi:10.1016/j.bbrc.2018.02.203.
APA：
Dongjie Liu,Mingyang Liu,Wenjing Wang,Lijun Pang,Zhenchang Wang...&Kai Liu.(2018).Overexpression of apoptosis-inducing factor mitochondrion-associated 1 (AIFM1) induces apoptosis by promoting the transcription of caspase3 and DRAM in hepatoma cells.BIOCHEMICAL and BIOPHYSICAL RESEARCH COMMUNICATIONS,498,(3)
MLA：
Dongjie Liu,et al."Overexpression of apoptosis-inducing factor mitochondrion-associated 1 (AIFM1) induces apoptosis by promoting the transcription of caspase3 and DRAM in hepatoma cells".BIOCHEMICAL and BIOPHYSICAL RESEARCH COMMUNICATIONS 498..3(2018):453-457
